Towards an understanding of wheat chloroplasts: A methodical investigation of thylakoid proteome

Abu Hena Mostafa Kamal, Kun Cho, Setsuko Komatsu, Nobuyuki Uozumi, Jong Soon Choi, Sun Hee Woo

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We utilized Percoll density gradient centrifugation to isolate and fractionate chloroplasts of Korean winter wheat cultivar cv. Kumgang (Triticum aestivum L.). The resulting protein fractions were separated by one dimensional polyacrylamide gel electrophoresis (1D-PAGE) coupled with LTQ-FTICR mass spectrometry. This enabled us to detect and identify 767 unique proteins. Our findings represent the most comprehensive exploration of a proteome to date. Based on annotation information from the UniProtKB/Swiss-Prot database and our analyses via WoLF PSORT and PSORT, these proteins are localized in the chloroplast (607 proteins), chloroplast stroma (145), thylakoid membrane (342), lumens (163), and integral membranes (166). In all, 67% were confirmed as chloroplast thylakoid proteins. Although nearly complete protein coverage (89% proteins) has been accomplished for the key chloroplast pathways in wheat, such as for photosynthesis, many other proteins are involved in regulating carbon metabolism. The identified proteins were assigned to 103 functional categories according to a classification system developed by the iProClass database and provided through Protein Information Resources. Those functions include electron transport, energy, cellular organization and biogenesis, transport, stress responses, and other metabolic processes. Whereas most of these proteins are associated with known complexes and metabolic pathways, about 13% of the proteins have unknown functions. The chloroplast proteome contains many proteins that are localized to the thylakoids but as yet have no known function. We propose that some of these familiar proteins participate in the photosynthetic pathway. Thus, our new and comprehensive protein profile may provide clues for better understanding that photosynthetic process in wheat.

Original languageEnglish
Pages (from-to)5069-5083
Number of pages15
JournalMolecular Biology Reports
Volume39
Issue number5
DOIs
Publication statusPublished - 2012 May 1

Keywords

  • LTQ-FTICR-MS
  • Proteomics
  • Thylakoid
  • Wheat chloroplast

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Towards an understanding of wheat chloroplasts: A methodical investigation of thylakoid proteome'. Together they form a unique fingerprint.

  • Cite this