Toward multi-analyte bioarray sensors: LAPS-based on-chip determination of a Michaelis-Menten-like kinetics for cell culturing

S. Takenaga, C. Herrera, C. F. Werner, M. Biselli, V. Thönnessen, Th Schnitzler, P. Öhlschläger, F. N. Almajhdi, T. Wagner, M. J. Schöning

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The metabolic activity of Chinese hamster ovary (CHO) cells was observed using a light-addressable potentiometric sensor (LAPS). The dependency toward different glucose concentrations (17-200 mM) follows a Michaelis-Menten kinetics trajectory with Km = 32.8 mM, and the obtained Km value in this experiment was compared with that found in literature. In addition, the pH shift induced by glucose metabolism of tumor cells transfected with the HPV-16 genome (C3 cells) was successfully observed. These results indicate the possibility to determine the tumor cells metabolism with a LAPS-based measurement device.

Original languageEnglish
Pages (from-to)1410-1415
Number of pages6
JournalPhysica Status Solidi (A) Applications and Materials Science
Volume211
Issue number6
DOIs
Publication statusPublished - 2014 Jun
Externally publishedYes

Keywords

  • Chinese hamster ovaries
  • cell metabolism
  • human papillomaviruses
  • light-addressable potentiometric sensors
  • tumor cells

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Electrical and Electronic Engineering
  • Materials Chemistry

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