Torque-speed curve of the Na+-driven flagellar motor of vibrio alginolyticus

Akihiko Ishijima, Yoshiyuki Sowa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The torque-speed relationship of the Na+-driven flagellar motor of Vibrio alginolyticus was investigated. The rotation rate of the motor was measured by following the position of a bead, attached to a flagellar filament, using optical nanometry. In the presence of 50 mM NaCl, the generated torque was relatively constant (∼3800 pN nm) at lower speeds (speeds up to ∼300 Hz) and then decreased steeply, similar to the H+-driven flagellar motor of E. coli. When the external NaCl concentration was varied, the generated torque of the flagellar motor was changed over a wide range of speeds. This result could be reproduced using a simple kinetic model, which takes into consideration the association and dissociation of Na+ onto the motor. These results imply that for a complete understanding of the mechanism of flagellar rotation it is essential to consider both the electrochemical gradient and the absolute concentration of the coupling ion.

Original languageEnglish
Title of host publication2005 5th IEEE Conference on Nanotechnology
Pages655-656
Number of pages2
DOIs
Publication statusPublished - 2005 Dec 1
Event2005 5th IEEE Conference on Nanotechnology - Nagoya, Japan
Duration: 2005 Jul 112005 Jul 15

Publication series

Name2005 5th IEEE Conference on Nanotechnology
Volume2

Other

Other2005 5th IEEE Conference on Nanotechnology
CountryJapan
CityNagoya
Period05/7/1105/7/15

ASJC Scopus subject areas

  • Engineering(all)

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    Ishijima, A., & Sowa, Y. (2005). Torque-speed curve of the Na+-driven flagellar motor of vibrio alginolyticus. In 2005 5th IEEE Conference on Nanotechnology (pp. 655-656). [1500801] (2005 5th IEEE Conference on Nanotechnology; Vol. 2). https://doi.org/10.1109/NANO.2005.1500801