Topoisomerase IIα expression in esophageal squamous cell carcinoma

Yusuke Ohashi, Hironobu Sasano, Hideo Yamaki, Soichiro Shizawa, Akihiko Kikuchi, Ryuzaburo Shineha, Takashi Akaishi, Susumu Satomi, Hiroshi Nagura

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


Background: DNA topoiseomerase IIα (topo IIα) is associated with active cell proliferation of mammalian cells. Topo IIα overexpression has been reported in a number of human malignancies and is considered to be related to their biological behaviors. Materials and Methods: We examined the expression of topo IIα immunohistochemically in 136 cases of human esophageal squamous cell carcinoma, 10 foci of squamous dysplasia and 10 non-pathologic squamous epithelium. We calculated the labeling index (LI) or the percentage of immunopositive cells for Topo IIα and Ki67, and Topo IIαLI/Ki67 LI (T/K ratio). These findings were then correlated with clinicopathological features of the patients including their clinical outcome. Results: Both topo IIα and Ki67 immunoreactivity were detected in the nuclei. A significant positive correlation was obtained between Topo IIα and Ki67 LIs in all the specimens examined. Topo IIα LI and T/K ratio were 24.5 ± 8.0% and 1.04 ± 0.64 for carcinoma, 19.1 ± 15.2% and 0.68 ± 0.29% for dysplasia and 14.0 ± 14.1% and 0.55 ± 0.17 for non-pathologic epithelium, respectively. Topo IIα LI and T/K ratio in carcinoma cases were significantly higher than those of normal epithelium. Topo IIα LI alone did not correlate with any of clinicopathological parameters examined but among carcinoma cases, cases with lymph nodes metastasis or higher histological stages had significantly higher T/K ratio than those without lymph node metastasis or lower histological stages. In addition, carcinoma cases with T/K ratio of greater than 0.8 demonstrated significantly worse prognosis than those with T/K ratio of smaller than 0.8. Conclusions: The relative overexpression of topo IIα as compared with Ki67, i.e. increased T/K ratio was detected in esophageal squamous cell carcinoma and is considered to represent a dysregulation or qualitative alteration in topo IIα, possibly associated with malignancies, as reported in other human cancers. In addition, topo IIα overexpression may also be correlated with the aggressive biological behavior of the patients with esophageal squamous cell carcinoma.

Original languageEnglish
Pages (from-to)1873-1880
Number of pages8
JournalAnticancer research
Issue number3 A
Publication statusPublished - 1999 Aug 30


  • Cell proliferation
  • Esophagus
  • Ki67
  • Squamous cell carcinoma
  • Topoisomerase IIα

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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