Topography and Permeability Analyses of Vasculature-on-a-Chip Using Scanning Probe Microscopies

Yuji Nashimoto, Minori Abe, Ryota Fujii, Noriko Taira, Hiroki Ida, Yasufumi Takahashi, Kosuke Ino, Javier Ramon-Azcon, Hitoshi Shiku

Research output: Contribution to journalArticlepeer-review

Abstract

Microphysiological systems (MPS) or organs-on-chips (OoC) can emulate the physiological functions of organs in vitro and are effective tools for determining human drug responses in preclinical studies. However, the analysis of MPS has relied heavily on optical tools, resulting in difficulties in real-time and high spatial resolution imaging of the target cell functions. In this study, the role of scanning probe microscopy (SPM) as an analytical tool for MPS is evaluated. An access hole is made in a typical MPS system with stacked microchannels to insert SPM probes into the system. For the first study, a simple vascular model composed of only endothelial cells is prepared for SPM analysis. Changes in permeability and local chemical flux are quantitatively evaluated during the construction of the vascular system. The morphological changes in the endothelial cells after flow stimulation are imaged at the single-cell level for topographical analysis. Finally, the possibility of adapting the permeability and topographical analysis using SPM for the intestinal vascular system is further evaluated. It is believed that this study will pave the way for an in situ permeability assay and structural analysis of MPS using SPM.

Original languageEnglish
Article number2101186
JournalAdvanced Healthcare Materials
Volume10
Issue number21
DOIs
Publication statusPublished - 2021 Nov 3
Externally publishedYes

Keywords

  • microphysiological systems
  • organs-chips
  • scanning electrochemical microscopy
  • scanning ion conductance microscopy
  • scanning probe microscopy

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Pharmaceutical Science

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