Topographic imaging of convoluted surface of live cells by scanning ion conductance microscopy in a standing approach mode

Yasufumi Takahashi, Yumi Murakami, Kuniaki Nagamine, Hitoshi Shiku, Shigeo Aoyagi, Tomoyuki Yasukawa, Makoto Kanzaki, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

78 Citations (Scopus)

Abstract

Scanning ion conductance microscopy (SICM) using a nanopipette as a probe and ionic current as a feedback signal was introduced as a novel technique to study live cells in a physiological environment. To avoid contact between the pipette tip and cells during the conventional lateral scanning mode, we adopted a standing approach (STA) mode in which the probe was moved vertically to first approach and then retracted from the cell surface at each measurement point on an XY plane. The STA mode ensured non-contact imaging of the topography of live cells and for a wide range of uneven substrates (500 × 300 µm to 5 × 5 µm). We also used a field-programmable gate array (FPGA) board to enhance feedback distance regulation. FPGA dramatically increased the feedback speed and decreased the imaging time (450 s per image) with enhanced accuracy and quality of live cell images. To evaluate the potential of the STA mode for SICM, we carried out imaging of a convoluted surface of live cell in various scan ranges and estimated the spatial resolutions of these images.

Original languageEnglish
Pages (from-to)10012-10017
Number of pages6
JournalPhysical Chemistry Chemical Physics
Volume12
Issue number34
DOIs
Publication statusPublished - 2010

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

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