Tonoplast H+-ATPase of mature pear fruit

Masaharu Hosaka; Yoshinori Kanayama; Katsuhiro Shiratake; Shohei Yamaki

doi:10.1016/S0031-9422(00)89775-7

Tonoplast H⁺-ATPase of mature pear fruit

Masaharu Hosaka, Yoshinori Kanayama, Katsuhiro Shiratake, Shohei Yamaki

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

Vacuolar type H⁺-ATPase (V-type ATPase) was isolated from a tonoplast preparation of mature pear Pyrus communis L. var. sativa DC, cv 'La France') fruit. This ATPase comprised 10 polypeptides of M_r 68 000, 54 000, 44 000, 42 000, 35 000, 30 000, 27 000, 16 000, 13 000 and 12 000 as subunits by SDS-PAGE. This ATPase was similar to the general V-type ATPase.

Original language	English
Pages (from-to)	565-567
Number of pages	3
Journal	Phytochemistry
Volume	36
Issue number	3
DOIs	https://doi.org/10.1016/S0031-9422(00)89775-7
Publication status	Published - 1994 Jun
Externally published	Yes

Keywords

H-ATPase
Pyrus communis
Rosaceae
V-type ATPase
enzyme purification
pear fruit
tonoplast.

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Plant Science
Horticulture

Access to Document

10.1016/S0031-9422(00)89775-7

Cite this

@article{2aeae6d6e3754ae7b39c3f1290c7a7e7,

title = "Tonoplast H+-ATPase of mature pear fruit",

abstract = "Vacuolar type H+-ATPase (V-type ATPase) was isolated from a tonoplast preparation of mature pear Pyrus communis L. var. sativa DC, cv 'La France') fruit. This ATPase comprised 10 polypeptides of Mr 68 000, 54 000, 44 000, 42 000, 35 000, 30 000, 27 000, 16 000, 13 000 and 12 000 as subunits by SDS-PAGE. This ATPase was similar to the general V-type ATPase.",

keywords = "H-ATPase, Pyrus communis, Rosaceae, V-type ATPase, enzyme purification, pear fruit, tonoplast.",

author = "Masaharu Hosaka and Yoshinori Kanayama and Katsuhiro Shiratake and Shohei Yamaki",

note = "Funding Information: Acknowledgements-We thank Dr M. Maeshimao f the Institute Low TemperatureS cience,H okkaido University for supplyinga ntibodiesa gainstm ung bean V-type ATPase. The researchr eportedi n this paper was supported in part by Grants-in-Aid (No. 02261102a nd 03304005f)r om the Ministry of Education,S ciencea nd Culture of Japan.",

year = "1994",

month = jun,

doi = "10.1016/S0031-9422(00)89775-7",

language = "English",

volume = "36",

pages = "565--567",

journal = "Phytochemistry",

issn = "0031-9422",

publisher = "Elsevier Limited",

number = "3",

}

TY - JOUR

T1 - Tonoplast H+-ATPase of mature pear fruit

AU - Hosaka, Masaharu

AU - Kanayama, Yoshinori

AU - Shiratake, Katsuhiro

AU - Yamaki, Shohei

N1 - Funding Information: Acknowledgements-We thank Dr M. Maeshimao f the Institute Low TemperatureS cience,H okkaido University for supplyinga ntibodiesa gainstm ung bean V-type ATPase. The researchr eportedi n this paper was supported in part by Grants-in-Aid (No. 02261102a nd 03304005f)r om the Ministry of Education,S ciencea nd Culture of Japan.

PY - 1994/6

Y1 - 1994/6

N2 - Vacuolar type H+-ATPase (V-type ATPase) was isolated from a tonoplast preparation of mature pear Pyrus communis L. var. sativa DC, cv 'La France') fruit. This ATPase comprised 10 polypeptides of Mr 68 000, 54 000, 44 000, 42 000, 35 000, 30 000, 27 000, 16 000, 13 000 and 12 000 as subunits by SDS-PAGE. This ATPase was similar to the general V-type ATPase.

AB - Vacuolar type H+-ATPase (V-type ATPase) was isolated from a tonoplast preparation of mature pear Pyrus communis L. var. sativa DC, cv 'La France') fruit. This ATPase comprised 10 polypeptides of Mr 68 000, 54 000, 44 000, 42 000, 35 000, 30 000, 27 000, 16 000, 13 000 and 12 000 as subunits by SDS-PAGE. This ATPase was similar to the general V-type ATPase.

KW - H-ATPase

KW - Pyrus communis

KW - Rosaceae

KW - V-type ATPase

KW - enzyme purification

KW - pear fruit

KW - tonoplast.

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DO - 10.1016/S0031-9422(00)89775-7

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SN - 0031-9422

VL - 36

SP - 565

EP - 567

JO - Phytochemistry

JF - Phytochemistry

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