TY - JOUR
T1 - Toll-like receptor 4 and cytokine expression involved in functional immune response in an originally established porcine intestinal epitheliocyte cell line
AU - Moue, Masayuki
AU - Tohno, Masanori
AU - Shimazu, Tomoyuki
AU - Kido, Taketomo
AU - Aso, Hisashi
AU - Saito, Tadao
AU - Kitazawa, Haruki
N1 - Funding Information:
We sincerely thank Dr. T. Shimosato (Center for Cancer Research, National Cancer Institute, USA) for helpful discussions and Dr. M. Nakazawa (National Institute of Animal Health, Japan) for the kind gift of ETEC strain 987P. This study was partly supported by a Grant-in-Aid for Scientific Research (B)(2) (No, 18380158) from the Japan Society for the Promotion of Science, an NISR Research Grant from the Noda Institute for Scientific Research to Dr. H. Kitazawa, and grants from the Secure and Healthy Animal Production project of the Ministry of Agriculture, Forestry and Fisheries of Japan to Dr. H. Aso and Dr. H. Kitazawa. M. Tohno was supported by a JSPS research fellowship (Research Fellowships for Young Scientists Program).
PY - 2008/2
Y1 - 2008/2
N2 - To study the immune responses of porcine intestinal epithelial cells to gram-negative bacteria via toll-like receptors (TLRs), originally established porcine intestinal epitheliocyte (PIE) cells were treated with lipopolysaccharide (LPS) or swine-specific enterotoxigenic Escherichia coli (ETEC). Real-time quantitative PCR revealed that PIE cells expressed TLR1-9 and MD-2 mRNAs, preferentially expressed TLR4/MD-2. Immunostaining of PIE cells revealed that TLR4 was precisely expressed in PIE cells at the protein level. PIE cells treated with LPS had up-regulated expression of several TLRs (TLR2, 3, 4, 5 and 8), type 1 helper T (Th1) cytokines (interleukin (IL)-1α, IL-1β, IL-6, IL-15, 18, leukemia inhibitory factor (LIF), and interferon (IFN)-β), and chemokines (monocyte chemoattractant protein (MCP)-1 and IL-8). ETEC enhanced the expression of TLR2, Th1 type cytokines (IL-1α, IL-12p35 and IL-6) and chemokines (MCP-1 and IL-8). These results indicate that PIE induces inflammatory responses by up-regulating Th1 cytokines and chemokines in response to LPS or ETEC, suggesting that PIE is a useful cell line for studying inflammatory responses via TLR4/MD-2 in intestinal epithelial cells.
AB - To study the immune responses of porcine intestinal epithelial cells to gram-negative bacteria via toll-like receptors (TLRs), originally established porcine intestinal epitheliocyte (PIE) cells were treated with lipopolysaccharide (LPS) or swine-specific enterotoxigenic Escherichia coli (ETEC). Real-time quantitative PCR revealed that PIE cells expressed TLR1-9 and MD-2 mRNAs, preferentially expressed TLR4/MD-2. Immunostaining of PIE cells revealed that TLR4 was precisely expressed in PIE cells at the protein level. PIE cells treated with LPS had up-regulated expression of several TLRs (TLR2, 3, 4, 5 and 8), type 1 helper T (Th1) cytokines (interleukin (IL)-1α, IL-1β, IL-6, IL-15, 18, leukemia inhibitory factor (LIF), and interferon (IFN)-β), and chemokines (monocyte chemoattractant protein (MCP)-1 and IL-8). ETEC enhanced the expression of TLR2, Th1 type cytokines (IL-1α, IL-12p35 and IL-6) and chemokines (MCP-1 and IL-8). These results indicate that PIE induces inflammatory responses by up-regulating Th1 cytokines and chemokines in response to LPS or ETEC, suggesting that PIE is a useful cell line for studying inflammatory responses via TLR4/MD-2 in intestinal epithelial cells.
KW - Cytokine
KW - Intestinal epitheliocyte
KW - Porcine
KW - Toll-like receptor
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U2 - 10.1016/j.bbagen.2007.11.006
DO - 10.1016/j.bbagen.2007.11.006
M3 - Article
C2 - 18082146
AN - SCOPUS:38549090164
VL - 1780
SP - 134
EP - 144
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
SN - 0006-3002
IS - 2
ER -