Toll-like receptor 4 and cytokine expression involved in functional immune response in an originally established porcine intestinal epitheliocyte cell line

Masayuki Moue, Masanori Tohno, Tomoyuki Shimazu, Taketomo Kido, Hisashi Aso, Tadao Saito, Haruki Kitazawa

Research output: Contribution to journalArticlepeer-review

98 Citations (Scopus)

Abstract

To study the immune responses of porcine intestinal epithelial cells to gram-negative bacteria via toll-like receptors (TLRs), originally established porcine intestinal epitheliocyte (PIE) cells were treated with lipopolysaccharide (LPS) or swine-specific enterotoxigenic Escherichia coli (ETEC). Real-time quantitative PCR revealed that PIE cells expressed TLR1-9 and MD-2 mRNAs, preferentially expressed TLR4/MD-2. Immunostaining of PIE cells revealed that TLR4 was precisely expressed in PIE cells at the protein level. PIE cells treated with LPS had up-regulated expression of several TLRs (TLR2, 3, 4, 5 and 8), type 1 helper T (Th1) cytokines (interleukin (IL)-1α, IL-1β, IL-6, IL-15, 18, leukemia inhibitory factor (LIF), and interferon (IFN)-β), and chemokines (monocyte chemoattractant protein (MCP)-1 and IL-8). ETEC enhanced the expression of TLR2, Th1 type cytokines (IL-1α, IL-12p35 and IL-6) and chemokines (MCP-1 and IL-8). These results indicate that PIE induces inflammatory responses by up-regulating Th1 cytokines and chemokines in response to LPS or ETEC, suggesting that PIE is a useful cell line for studying inflammatory responses via TLR4/MD-2 in intestinal epithelial cells.

Original languageEnglish
Pages (from-to)134-144
Number of pages11
JournalBiochimica et Biophysica Acta - General Subjects
Volume1780
Issue number2
DOIs
Publication statusPublished - 2008 Feb 1

Keywords

  • Cytokine
  • Intestinal epitheliocyte
  • Porcine
  • Toll-like receptor

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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