Toll-like receptor 2 (TLR2) and dectin-1 contribute to the production of IL-12p40 by bone marrow-derived dendritic cells infected with Penicillium marneffei

Kiwamu Nakamura, Akiko Miyazato, Yoshinobu Koguchi, Yoshiyuki Adachi, Naohito Ohno, Shinobu Saijo, Yoichiro Iwakura, Kiyoshi Takeda, Shizuo Akira, Jiro Fujita, Keiko Ishii, Mitsuo Kaku, Kazuyoshi Kawakami

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

The present study was designed to elucidate the role of TLR2, TLR4 and dectin-1 in the production of IL-12p40 by bone marrow-derived dendritic cells (BM-DCs) infected with Penicillium marneffei. IL-12p40 production was almost completely abrogated in BM-DCs from TLR2 gene-knockout (KO) and MyD88KO mice, but not from TLR4-defective C3H/HeJ mice compared to those from control mice. Furthermore, BM-DCs from dectin-1KO mice faintly produced IL-12p40 upon stimulation with this fungus. Using a luciferase reporter assay, P. marneffei activated NF-κB in HEK293 cells transfected with the TLR2 gene, but not with the dectin-1 gene, and their co-transfection did not lead to further increase in this response. These results indicate that TLR2 and dectin-1 are essential in sensing P. marneffei for the activation of BM-DCs.

Original languageEnglish
Pages (from-to)1223-1227
Number of pages5
JournalMicrobes and Infection
Volume10
Issue number10-11
DOIs
Publication statusPublished - 2008 Aug

Keywords

  • Dectin-1
  • Dendritic cells
  • Interleukin-12
  • MyD88
  • Penicillium marneffei
  • Toll-like receptor 2

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Infectious Diseases

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