Tol2-mediated transgenesis, gene trapping, enhancer trapping, and the GAL4-UAS system

Gembu Abe, Maximilliano L. Suster, Koichi Kawakami

Research output: Chapter in Book/Report/Conference proceedingChapter

26 Citations (Scopus)

Abstract

The Tol2 transposable element was originally found in the genome of the Japanese medaka fish (Oryzias latipes). Tol2 contains a gene encoding an active transposase that can catalyze DNA transposition in vertebrate cells. In zebrafish, Tol2 generates genomic integrations in the germ cells very efficiently. By using the Tol2 transposition system, we have developed important genetic methods including transgenesis, gene trapping, enhancer trapping, and the Gal4-UAS system in zebrafish. In this chapter, we describe how these methods can be performed.

Original languageEnglish
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc.
Pages23-49
Number of pages27
DOIs
Publication statusPublished - 2011
Externally publishedYes

Publication series

NameMethods in Cell Biology
Volume104
ISSN (Print)0091-679X

Keywords

  • Enhancer
  • Gene trapping
  • Germline
  • Microinjection
  • Transgenesis
  • Transposable

ASJC Scopus subject areas

  • Cell Biology

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