@inbook{2e6494125a3d439881f979c04b350f02,
title = "Tol2-mediated transgenesis, gene trapping, enhancer trapping, and the GAL4-UAS system",
abstract = "The Tol2 transposable element was originally found in the genome of the Japanese medaka fish (Oryzias latipes). Tol2 contains a gene encoding an active transposase that can catalyze DNA transposition in vertebrate cells. In zebrafish, Tol2 generates genomic integrations in the germ cells very efficiently. By using the Tol2 transposition system, we have developed important genetic methods including transgenesis, gene trapping, enhancer trapping, and the Gal4-UAS system in zebrafish. In this chapter, we describe how these methods can be performed.",
keywords = "Enhancer, Gene trapping, Germline, Microinjection, Transgenesis, Transposable",
author = "Gembu Abe and Suster, {Maximilliano L.} and Koichi Kawakami",
note = "Funding Information: We express our gratitude for support from the Sasakawa scientific research grant of the Japan Science Society (to GA), the National BioResource Project, and grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan.",
year = "2011",
doi = "10.1016/B978-0-12-374814-0.00002-1",
language = "English",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc.",
pages = "23--49",
booktitle = "Methods in Cell Biology",
}