TOF-SIMS investigation of metallic material surface after culturing cells

Satoka Aoyagi, Sachiko Hiromoto, Takao Hanawa, Masahiro Kudo

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Biomolecules such as extracellular matrix and adhesive proteins generated by adhered cells on metallic specimens were characterized by means of time-of-flight secondary ion mass spectrometry (TOF-SIMS) in order to clarify the interaction between cells and metal surfaces. Since composition and structure of the extracellular matrix depends on conditions of cells, characterization of the interaction between cells and metallic specimens is important in order to evaluate the biocompatibility and the degradation behavior of metallic biomaterials and artificial organs. Moreover, the obtained data can contribute to the development of new metallic biomaterials. TOF-SIMS spectra were analyzed by means of mutual information described by information theory and principal components analysis (PCA). The results show that cells have great influence on adsorption of biomolecules on metallic materials because they change surface conditions of the materials. Thus TOF-SIMS is a useful technique to investigate the interaction between metallic biomaterials and cells.

Original languageEnglish
Pages (from-to)470-474
Number of pages5
JournalApplied Surface Science
Publication statusPublished - 2004 Jun 15
Externally publishedYes


  • Cell
  • Extracellular matrix
  • Information theory
  • Mutual information
  • Principal component analysis

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics
  • Physics and Astronomy(all)
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films


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