Tobacco ZFT1, a transcriptional repressor with a Cys2/His 2 type zinc finger motif that functions in spermine-signaling pathway

Yukiko Uehara, Yoshihiro Takahashi, Thomas Berberich, Atsushi Miyazaki, Hideki Takahashi, Kyoko Matsui, Masaru Ohme-Takagi, Hiromasa Saitoh, Ryohei Terauchi, Tomonobu Kusano

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

We previously proposed that a spermine (Spm)-mediated signal transduction pathway is involved in the hypersensitive response induced by Tobacco mosaic virus (TMV) in tobacco plants. To identify regulatory component(s) of this pathway, we surveyed a tobacco cDNA library and found that the ZFT1 gene, which encodes a Cys2/His2 type zinc-finger protein, is Spm-responsive. ZFT1 was not induced by two other polyamines, putrescine and spermidine, or by salicylic acid (SA), jasmonic acid or ethylene. Furthermore, ZFT1 was upregulated in TMV- inoculated tobacco plants in an N gene-dependent manner. Notably, induction of ZFT1 by Spm and by TMV infection was unimpaired in NahG-transgenic tobacco plants, indicating that cross-talk with an SA signaling pathway is not involved in this response. Within the Spm-signaling pathway, we found that ZFT1 functioned downstream of both mitochondrial dysfunction and mitogen-activated protein kinase activation. The ZFT1 protein has two zinc finger motifs and shows a high degree of similarity to ZPT2-3 in petunia and SCOF1 in soybean. However, unlike the latter two proteins, ZFT1 binds to the EP1S sequence and functions as a transcription repressor. Moreover, interestingly, ZFT1 overexpression rendered tobacco plants more tolerant to TMV. Based on the results presented here, we propose that ZFT1 functions as a transcription repressor in a Spm signaling pathway, thereby accelerating necrotic local region formation in tobacco leaves.

Original languageEnglish
Pages (from-to)435-448
Number of pages14
JournalPlant Molecular Biology
Volume59
Issue number3
DOIs
Publication statusPublished - 2005 Oct

Keywords

  • Signal transduction
  • Spermine
  • Tobacco
  • Tobacco mosaic virus
  • Transcription factor
  • Zinc finger protein

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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