TNF-α stimulates the expression of RANK during orthodontic tooth movement

Takahiro Noguchi, Hideki Kitaura, Saika Ogawa, Jiawei Qi, Wei Ren Shen, Fumitoshi Ohori, Aseel Marahleh, Yasuhiko Nara, Adya Pramusita, Itaru Mizoguchi

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Objective: This study aimed to evaluate the effects of tumor necrosis factor (TNF)-α on receptor activator of nuclear factor-κB (RANK) expression in osteoclast precursors in vitro and during orthodontic tooth movement (OTM) in vivo. Design: We assessed whether TNF-α influenced RANK expression levels in osteoclast precursors in vitro by real-time PCR and western blotting. For in vivo experiments, TNF-α was subcutaneously injected into mouse calvariae daily for 5 days. Mice were sacrificed and RANK expression was evaluated by real-time PCR and immunohistochemistry. For OTM, a nickel-titanium closed-coil spring was fixed between the upper incisors and upper-left first molar to move the first molar in the mesial direction in wild-type (WT) and TNFR1/TNFR2-deficient (TNFRsKO) mice. After OTM, the number of RANK-positive cells on the compression side was evaluated by immunohistochemistry. Results: RANK levels were enhanced in TNF-α-treated osteoclast precursors in vitro. RANK mRNA expression levels and the number of RANK-positive cells were higher in TNF-α-injected mice than in phosphate-buffered saline-injected mice. RANK-positive cells increased on the compression side of the alveolar bone in WT mice because of the mechanical loading. In addition, the number of RANK-positive cells on the compression side was significantly higher in WT mice than in TNFRsKO mice after OTM. Conclusion: These results suggest that TNF-α induces RANK expression in vitro and at baseline in vivo, as well as on the compression side during OTM.

Original languageEnglish
Article number104796
JournalArchives of Oral Biology
Volume117
DOIs
Publication statusPublished - 2020 Sep

Keywords

  • Mouse
  • Orthodontic tooth movement
  • Osteoclast
  • RANK
  • TNF-α

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

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