Tissue Prx I in the protection against Fe-NTA and the reduction of nitroxyl radicals

Junya Uwayama, Aki Hirayama, Toru Yanagawa, Eiji Warabi, Rika Sugimoto, Ken Itoh, Masayuki Yamamoto, Hiroshi Yoshida, Akio Koyama, Tetsuro Ishii

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Peroxiredoxin I (Prx I) is a key cytoplasmic peroxidase that reduces intracellular hydroperoxides in concert with thioredoxin. To study the role of tissue Prx I in protection from oxidative stress, we generated Prx I -/- mice by gene trapping. We then evaluated the acute-phase tissue damage caused by ferric-nitrilotriacetate (Fe-NTA). Increases in serum aspartate aminotransferase and alanine aminotransferase levels were significantly greater in Prx I-/- than wild-type mice, 4 and 12 h after the injection of Fe-NTA. Using real-time EPR imaging, we examined the reduction of the stable paramagnetic nitroxyl radical 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl in vivo, and found that the half-life of this spin probe in the liver and kidney was significantly prolonged in the Prx I-/- mice. These results demonstrate that Prx I-/- mice have less reducing activity and are more susceptible to the damage mediated by reactive oxygen species in vivo than wild-type mice.

Original languageEnglish
Pages (from-to)226-231
Number of pages6
JournalBiochemical and biophysical research communications
Volume339
Issue number1
DOIs
Publication statusPublished - 2006 Jan 6
Externally publishedYes

Keywords

  • Carbamoyl-PROXYL
  • EPR imaging
  • Ferric-nitrilotriacetate
  • Free radicals
  • OmniBank
  • Peroxiredoxin I

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Tissue Prx I in the protection against Fe-NTA and the reduction of nitroxyl radicals'. Together they form a unique fingerprint.

Cite this