Tissue engineering of the small intestine by acellular collagen sponge scaffold grafting

Y. Hori, T. Nakamura, K. Matsumoto, Y. Kurokawa, S. Satomi, Y. Shimizu

Research output: Contribution to journalArticlepeer-review

57 Citations (Scopus)

Abstract

Tissue engineering of the small intestine will prove a great benefit to patients suffering from short bowel disease. However cell seeding in tissue engineering, such as fetal cell use, is accompanied by problems of ethical issues, rejection, and short supply. To overcome these problems, we carried out an experimental study on tissue engineering of the small intestine by acellular collagen sponge scaffold grafting. We resected the 5 cm long jejunum from beagle dogs and reconstructed it by acellular collagen sponge grafting with a silicon tube stent. The graft was covered with the omentum. At 1 month after operation, the silicon stent was removed endoscopically. Animals were sacrificed 1 and 4 months after operation, and were examined microscopically. Neo-intestinal regeneration was observed and the intestinal mucosa covered the luminal side of the regenerated intestine across the anastomosis. Thus, the small intestine was regenerated by tissue engineering technology using an acellular collagen sponge scaffold.

Original languageEnglish
Pages (from-to)50-54
Number of pages5
JournalInternational Journal of Artificial Organs
Volume24
Issue number1
Publication statusPublished - 2001 Mar 3

Keywords

  • Collagen
  • Intestine
  • Regeneration
  • Scaffold
  • Tissue engineering

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

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