The C-terminal α-amide formation of peptides is one of the most important events in prohormone processing. Recently, we developed a simple and sensitive assay for detecting α-amidating activity in tissues by using (125I)-Ac-Tyr-Phe-Gly as a substrate. Using this assay method, we have determined the tissue distribution of α-amidating enzyme activity in adult male rat. High concentrations of α-amidating activity were found in pituitary, brain, thyoid, gastrointestinal tract, pancreas, heart, submaxillary glands and parotid glands. Alpha-amidating enzyme activities in all tissues examined exhibit very similar copper and ascorbate requirements, pH dependence, and behavior on gel-filtration.
|Number of pages||7|
|Journal||Biochemical and biophysical research communications|
|Publication status||Published - 1986 Oct 15|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology