Tissue distribution and characterization of peptide C-terminal α-amidating activity in rat

Junichiro Sakata, Kensaku Mizuno, Hisayuki Matsuo

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)

Abstract

The C-terminal α-amide formation of peptides is one of the most important events in prohormone processing. Recently, we developed a simple and sensitive assay for detecting α-amidating activity in tissues by using (125I)-Ac-Tyr-Phe-Gly as a substrate. Using this assay method, we have determined the tissue distribution of α-amidating enzyme activity in adult male rat. High concentrations of α-amidating activity were found in pituitary, brain, thyoid, gastrointestinal tract, pancreas, heart, submaxillary glands and parotid glands. Alpha-amidating enzyme activities in all tissues examined exhibit very similar copper and ascorbate requirements, pH dependence, and behavior on gel-filtration.

Original languageEnglish
Pages (from-to)230-236
Number of pages7
JournalBiochemical and biophysical research communications
Volume140
Issue number1
DOIs
Publication statusPublished - 1986 Oct 15
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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