TY - JOUR
T1 - Time-kill kinetic analysis of antimicrobial chemotherapy based on hydrogen peroxide photolysis against Streptococcus mutans biofilm
AU - Shirato, Midori
AU - Nakamura, Keisuke
AU - Kanno, Taro
AU - Lingström, Peter
AU - Niwano, Yoshimi
AU - Örtengren, Ulf
N1 - Funding Information:
This study was supported by JSPS KAKENHI Grant-in-Aid for Young Scientists (B), [grant number 16K20531] and by the Ministry of Health, Labour and Welfare, Japan, and the “Tohoku Region Innovative Medical Devices Research and Development Project”. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/8
Y1 - 2017/8
N2 - A recently developed antimicrobial technique utilizing hydroxyl radicals generated by hydrogen peroxide (H2O2) photolysis represents a promising new therapy for preventing and treating dental caries. The present study compared the antimicrobial time-kill kinetics of H2O2 photolysis, conventional antiseptics, and antimicrobial photodynamic therapy (aPDT) against biofilm-forming Streptococcus mutans (cariogenic bacteria) grown on hydroxyapatite disks. H2O2 photolysis was performed by irradiating the biofilm immersed in 3% H2O2 with 365-nm light-emitting diode (LED) light at an irradiance of 1000 mW/cm2 for up to 1.5 min. Antiseptic treatments consisted of 0.2% chlorhexidine gluconate, 0.5% povidone-iodine, and 3% H2O2. The biofilm was immersed in each antiseptic for up to 4 min. aPDT was performed by irradiating the biofilm immersed in 100 μM methylene blue or toluidine blue O with 655-nm laser light at 1000 mW/cm2 for up to 4 min. Based on the time-kill assay, the decimal reduction value (D-value) of each treatment was determined. With a D-value of 0.06 min, H2O2 photolysis exhibited the highest bactericidal effect against biofilm-forming S. mutans. In contrast, antiseptics and aPDT exerted a slower bactericidal effect, with D-values of 0.9–2.7 min. In conclusion, the antimicrobial technique based on H2O2 photolysis using 365-nm LED represents a strong adjunctive chemotherapy for dental caries treatment.
AB - A recently developed antimicrobial technique utilizing hydroxyl radicals generated by hydrogen peroxide (H2O2) photolysis represents a promising new therapy for preventing and treating dental caries. The present study compared the antimicrobial time-kill kinetics of H2O2 photolysis, conventional antiseptics, and antimicrobial photodynamic therapy (aPDT) against biofilm-forming Streptococcus mutans (cariogenic bacteria) grown on hydroxyapatite disks. H2O2 photolysis was performed by irradiating the biofilm immersed in 3% H2O2 with 365-nm light-emitting diode (LED) light at an irradiance of 1000 mW/cm2 for up to 1.5 min. Antiseptic treatments consisted of 0.2% chlorhexidine gluconate, 0.5% povidone-iodine, and 3% H2O2. The biofilm was immersed in each antiseptic for up to 4 min. aPDT was performed by irradiating the biofilm immersed in 100 μM methylene blue or toluidine blue O with 655-nm laser light at 1000 mW/cm2 for up to 4 min. Based on the time-kill assay, the decimal reduction value (D-value) of each treatment was determined. With a D-value of 0.06 min, H2O2 photolysis exhibited the highest bactericidal effect against biofilm-forming S. mutans. In contrast, antiseptics and aPDT exerted a slower bactericidal effect, with D-values of 0.9–2.7 min. In conclusion, the antimicrobial technique based on H2O2 photolysis using 365-nm LED represents a strong adjunctive chemotherapy for dental caries treatment.
KW - Biofilm
KW - Chlorhexidine gluconate
KW - Hydrogen peroxide
KW - Hydroxyl radical
KW - Photodynamic therapy
KW - Streptococcus mutans
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U2 - 10.1016/j.jphotobiol.2017.06.023
DO - 10.1016/j.jphotobiol.2017.06.023
M3 - Article
C2 - 28666212
AN - SCOPUS:85021304990
VL - 173
SP - 434
EP - 440
JO - Journal of Photochemistry and Photobiology B: Biology
JF - Journal of Photochemistry and Photobiology B: Biology
SN - 1011-1344
ER -