Time-kill kinetic analysis of antimicrobial chemotherapy based on hydrogen peroxide photolysis against Streptococcus mutans biofilm

Midori Shirato, Keisuke Nakamura, Taro Kanno, Peter Lingström, Yoshimi Niwano, Ulf Örtengren

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

A recently developed antimicrobial technique utilizing hydroxyl radicals generated by hydrogen peroxide (H2O2) photolysis represents a promising new therapy for preventing and treating dental caries. The present study compared the antimicrobial time-kill kinetics of H2O2 photolysis, conventional antiseptics, and antimicrobial photodynamic therapy (aPDT) against biofilm-forming Streptococcus mutans (cariogenic bacteria) grown on hydroxyapatite disks. H2O2 photolysis was performed by irradiating the biofilm immersed in 3% H2O2 with 365-nm light-emitting diode (LED) light at an irradiance of 1000 mW/cm2 for up to 1.5 min. Antiseptic treatments consisted of 0.2% chlorhexidine gluconate, 0.5% povidone-iodine, and 3% H2O2. The biofilm was immersed in each antiseptic for up to 4 min. aPDT was performed by irradiating the biofilm immersed in 100 μM methylene blue or toluidine blue O with 655-nm laser light at 1000 mW/cm2 for up to 4 min. Based on the time-kill assay, the decimal reduction value (D-value) of each treatment was determined. With a D-value of 0.06 min, H2O2 photolysis exhibited the highest bactericidal effect against biofilm-forming S. mutans. In contrast, antiseptics and aPDT exerted a slower bactericidal effect, with D-values of 0.9–2.7 min. In conclusion, the antimicrobial technique based on H2O2 photolysis using 365-nm LED represents a strong adjunctive chemotherapy for dental caries treatment.

Original languageEnglish
Pages (from-to)434-440
Number of pages7
JournalJournal of Photochemistry and Photobiology B: Biology
Volume173
DOIs
Publication statusPublished - 2017 Aug

Keywords

  • Biofilm
  • Chlorhexidine gluconate
  • Hydrogen peroxide
  • Hydroxyl radical
  • Photodynamic therapy
  • Streptococcus mutans

ASJC Scopus subject areas

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology Nuclear Medicine and imaging

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