Changes in patterns of microfilament distribution were analyzed using cultured porcine aortic endothelial cells under shear flow. Within the elongated endothelial cells after shear flow exposure (20 dyne/cm2, 0-24 h), F-actin filaments were mainly observed as stress fibers. After 24 h exposure, the fluorescence intensity of rhodamine-phalloidin-stained cells was enhanced, indicating the increase in F-actin content. It was suggested that not only F-actin distribution but also the content may be affected by shear stress at the early stage of exposure.
|Number of pages||5|
|Journal||Frontiers of medical and biological engineering : the international journal of the Japan Society of Medical Electronics and Biological Engineering|
|Publication status||Published - 1993|
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