Tight linkage of the ret proto-oncogene with the multiple endocrine neoplasia type 2A locus

Masayuki Yamamoto; Tetsuro Miki; Norifumi Tanaka; Akihiro Miya; Eisei Shin; Katsu Karakawa; Tetsuro Kobayashi; Tomoko Tahira; Yukihito Ishizaka; Fumio Itoh; Minako Nagao; Takesada Mori; Shin Ichiro Takai

Tight linkage of the ret proto-oncogene with the multiple endocrine neoplasia type 2A locus

Masayuki Yamamoto, Tetsuro Miki, Norifumi Tanaka, Akihiro Miya, Eisei Shin, Katsu Karakawa, Tetsuro Kobayashi, Tomoko Tahira, Yukihito Ishizaka, Fumio Itoh, Minako Nagao, Takesada Mori, Shin Ichiro Takai

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

The ret proto-oncogene has been mapped to 10q11.2 near the MEN2A locus by in situ hybridization. We carried out a linkage study of Japanese multiple endocrine neoplasia type 2A (MEN2A) families using a cosmid clone containing the ret proto-oncogene as probe. Two polymorphic alleles (A1 and A2) could be detected by digesting DNA with EcoRl: allele A1 was detected as a 10 kb fragment and A2 as 5.4 and 4.6 kb fragments. Of 11 Japanese MEN2A families analysed, four were informative, and the maximum lod score was 4.23 at a recombination fraction of 0.00. This result suggests the ret proto-oncogene to be close to the MEN2A gene and therefore possibly to be a useful DNA marker for cloning the latter.

Original language	English
Pages (from-to)	149-152
Number of pages	4
Journal	Japanese journal of clinical oncology
Volume	21
Issue number	3
Publication status	Published - 1991 Jun 1
Externally published	Yes

Keywords

Linkage
Lod score
MEN2A
RELP (Restriction Fragment Length Polymorphism)
Ret proto-oncogene

ASJC Scopus subject areas

Oncology
Radiology Nuclear Medicine and imaging
Cancer Research

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Tight linkage of the ret proto-oncogene with the multiple endocrine neoplasia type 2A locus. / Yamamoto, Masayuki; Miki, Tetsuro; Tanaka, Norifumi; Miya, Akihiro; Shin, Eisei; Karakawa, Katsu; Kobayashi, Tetsuro; Tahira, Tomoko; Ishizaka, Yukihito; Itoh, Fumio; Nagao, Minako; Mori, Takesada; Takai, Shin Ichiro.

In: Japanese journal of clinical oncology, Vol. 21, No. 3, 01.06.1991, p. 149-152.

Research output: Contribution to journal › Article › peer-review

Yamamoto, Masayuki ; Miki, Tetsuro ; Tanaka, Norifumi ; Miya, Akihiro ; Shin, Eisei ; Karakawa, Katsu ; Kobayashi, Tetsuro ; Tahira, Tomoko ; Ishizaka, Yukihito ; Itoh, Fumio ; Nagao, Minako ; Mori, Takesada ; Takai, Shin Ichiro. / Tight linkage of the ret proto-oncogene with the multiple endocrine neoplasia type 2A locus. In: Japanese journal of clinical oncology. 1991 ; Vol. 21, No. 3. pp. 149-152.

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abstract = "The ret proto-oncogene has been mapped to 10q11.2 near the MEN2A locus by in situ hybridization. We carried out a linkage study of Japanese multiple endocrine neoplasia type 2A (MEN2A) families using a cosmid clone containing the ret proto-oncogene as probe. Two polymorphic alleles (A1 and A2) could be detected by digesting DNA with EcoRl: allele A1 was detected as a 10 kb fragment and A2 as 5.4 and 4.6 kb fragments. Of 11 Japanese MEN2A families analysed, four were informative, and the maximum lod score was 4.23 at a recombination fraction of 0.00. This result suggests the ret proto-oncogene to be close to the MEN2A gene and therefore possibly to be a useful DNA marker for cloning the latter.",

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AU - Shin, Eisei

AU - Karakawa, Katsu

AU - Kobayashi, Tetsuro

AU - Tahira, Tomoko

AU - Ishizaka, Yukihito

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AU - Nagao, Minako

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AU - Takai, Shin Ichiro

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AB - The ret proto-oncogene has been mapped to 10q11.2 near the MEN2A locus by in situ hybridization. We carried out a linkage study of Japanese multiple endocrine neoplasia type 2A (MEN2A) families using a cosmid clone containing the ret proto-oncogene as probe. Two polymorphic alleles (A1 and A2) could be detected by digesting DNA with EcoRl: allele A1 was detected as a 10 kb fragment and A2 as 5.4 and 4.6 kb fragments. Of 11 Japanese MEN2A families analysed, four were informative, and the maximum lod score was 4.23 at a recombination fraction of 0.00. This result suggests the ret proto-oncogene to be close to the MEN2A gene and therefore possibly to be a useful DNA marker for cloning the latter.

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