Abstract
The ret proto-oncogene has been mapped to 10q11.2 near the MEN2A locus by in situ hybridization. We carried out a linkage study of Japanese multiple endocrine neoplasia type 2A (MEN2A) families using a cosmid clone containing the ret proto-oncogene as probe. Two polymorphic alleles (A1 and A2) could be detected by digesting DNA with EcoRl: allele A1 was detected as a 10 kb fragment and A2 as 5.4 and 4.6 kb fragments. Of 11 Japanese MEN2A families analysed, four were informative, and the maximum lod score was 4.23 at a recombination fraction of 0.00. This result suggests the ret proto-oncogene to be close to the MEN2A gene and therefore possibly to be a useful DNA marker for cloning the latter.
Original language | English |
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Pages (from-to) | 149-152 |
Number of pages | 4 |
Journal | Japanese journal of clinical oncology |
Volume | 21 |
Issue number | 3 |
Publication status | Published - 1991 Jun |
Externally published | Yes |
Keywords
- Linkage
- Lod score
- MEN2A
- RELP (Restriction Fragment Length Polymorphism)
- Ret proto-oncogene
ASJC Scopus subject areas
- Oncology
- Radiology Nuclear Medicine and imaging
- Cancer Research