Tight linkage of the ret proto-oncogene with the multiple endocrine neoplasia type 2A locus

Masayuki Yamamoto; Tetsuro Miki; Norifumi Tanaka; Akihiro Miya; Eisei Shin; Katsu Karakawa; Tetsuro Kobayashi; Tomoko Tahira; Yukihito Ishizaka; Fumio Itoh; Minako Nagao; Takesada Mori; Shin Ichiro Takai

Tight linkage of the ret proto-oncogene with the multiple endocrine neoplasia type 2A locus

Masayuki Yamamoto, Tetsuro Miki, Norifumi Tanaka, Akihiro Miya, Eisei Shin, Katsu Karakawa, Tetsuro Kobayashi, Tomoko Tahira, Yukihito Ishizaka, Fumio Itoh, Minako Nagao, Takesada Mori, Shin Ichiro Takai

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

The ret proto-oncogene has been mapped to 10q11.2 near the MEN2A locus by in situ hybridization. We carried out a linkage study of Japanese multiple endocrine neoplasia type 2A (MEN2A) families using a cosmid clone containing the ret proto-oncogene as probe. Two polymorphic alleles (A1 and A2) could be detected by digesting DNA with EcoRl: allele A1 was detected as a 10 kb fragment and A2 as 5.4 and 4.6 kb fragments. Of 11 Japanese MEN2A families analysed, four were informative, and the maximum lod score was 4.23 at a recombination fraction of 0.00. This result suggests the ret proto-oncogene to be close to the MEN2A gene and therefore possibly to be a useful DNA marker for cloning the latter.

Original language	English
Pages (from-to)	149-152
Number of pages	4
Journal	Japanese journal of clinical oncology
Volume	21
Issue number	3
Publication status	Published - 1991 Jun
Externally published	Yes

Keywords

Linkage
Lod score
MEN2A
RELP (Restriction Fragment Length Polymorphism)
Ret proto-oncogene

ASJC Scopus subject areas

Oncology
Radiology Nuclear Medicine and imaging
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@article{dd658bf5985047ac8832581da635631f,

title = "Tight linkage of the ret proto-oncogene with the multiple endocrine neoplasia type 2A locus",

abstract = "The ret proto-oncogene has been mapped to 10q11.2 near the MEN2A locus by in situ hybridization. We carried out a linkage study of Japanese multiple endocrine neoplasia type 2A (MEN2A) families using a cosmid clone containing the ret proto-oncogene as probe. Two polymorphic alleles (A1 and A2) could be detected by digesting DNA with EcoRl: allele A1 was detected as a 10 kb fragment and A2 as 5.4 and 4.6 kb fragments. Of 11 Japanese MEN2A families analysed, four were informative, and the maximum lod score was 4.23 at a recombination fraction of 0.00. This result suggests the ret proto-oncogene to be close to the MEN2A gene and therefore possibly to be a useful DNA marker for cloning the latter.",

keywords = "Linkage, Lod score, MEN2A, RELP (Restriction Fragment Length Polymorphism), Ret proto-oncogene",

author = "Masayuki Yamamoto and Tetsuro Miki and Norifumi Tanaka and Akihiro Miya and Eisei Shin and Katsu Karakawa and Tetsuro Kobayashi and Tomoko Tahira and Yukihito Ishizaka and Fumio Itoh and Minako Nagao and Takesada Mori and Takai, {Shin Ichiro}",

note = "Funding Information: We thank Miss S. Fujita for technical assistance. This work was supported by a Grant-in-Aid for Cancer Research, from the Ministry of Education, Science and Culture of Japan.",

year = "1991",

month = jun,

language = "English",

volume = "21",

pages = "149--152",

journal = "Japanese Journal of Clinical Oncology",

issn = "0368-2811",

publisher = "Oxford University Press",

number = "3",

}

TY - JOUR

T1 - Tight linkage of the ret proto-oncogene with the multiple endocrine neoplasia type 2A locus

AU - Yamamoto, Masayuki

AU - Miki, Tetsuro

AU - Tanaka, Norifumi

AU - Miya, Akihiro

AU - Shin, Eisei

AU - Karakawa, Katsu

AU - Kobayashi, Tetsuro

AU - Tahira, Tomoko

AU - Ishizaka, Yukihito

AU - Itoh, Fumio

AU - Nagao, Minako

AU - Mori, Takesada

AU - Takai, Shin Ichiro

N1 - Funding Information: We thank Miss S. Fujita for technical assistance. This work was supported by a Grant-in-Aid for Cancer Research, from the Ministry of Education, Science and Culture of Japan.

PY - 1991/6

Y1 - 1991/6

N2 - The ret proto-oncogene has been mapped to 10q11.2 near the MEN2A locus by in situ hybridization. We carried out a linkage study of Japanese multiple endocrine neoplasia type 2A (MEN2A) families using a cosmid clone containing the ret proto-oncogene as probe. Two polymorphic alleles (A1 and A2) could be detected by digesting DNA with EcoRl: allele A1 was detected as a 10 kb fragment and A2 as 5.4 and 4.6 kb fragments. Of 11 Japanese MEN2A families analysed, four were informative, and the maximum lod score was 4.23 at a recombination fraction of 0.00. This result suggests the ret proto-oncogene to be close to the MEN2A gene and therefore possibly to be a useful DNA marker for cloning the latter.

AB - The ret proto-oncogene has been mapped to 10q11.2 near the MEN2A locus by in situ hybridization. We carried out a linkage study of Japanese multiple endocrine neoplasia type 2A (MEN2A) families using a cosmid clone containing the ret proto-oncogene as probe. Two polymorphic alleles (A1 and A2) could be detected by digesting DNA with EcoRl: allele A1 was detected as a 10 kb fragment and A2 as 5.4 and 4.6 kb fragments. Of 11 Japanese MEN2A families analysed, four were informative, and the maximum lod score was 4.23 at a recombination fraction of 0.00. This result suggests the ret proto-oncogene to be close to the MEN2A gene and therefore possibly to be a useful DNA marker for cloning the latter.

KW - Linkage

KW - Lod score

KW - MEN2A

KW - RELP (Restriction Fragment Length Polymorphism)

KW - Ret proto-oncogene

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M3 - Article

C2 - 1682518

AN - SCOPUS:0026176399

VL - 21

SP - 149

EP - 152

JO - Japanese Journal of Clinical Oncology

JF - Japanese Journal of Clinical Oncology

SN - 0368-2811

IS - 3

ER -

Tight linkage of the ret proto-oncogene with the multiple endocrine neoplasia type 2A locus

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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