Three-repeat Tau 69 is a major tau isoform in laser-microdissected Pick bodies

Takuya Ohkubo, Yuji Sakasegawa, Hiroyuki Toda, Hitaru Kishida, Kunimasa Arima, Mitsunori Yamada, Hitoshi Takahashi, Hidehiro Mizusawa, Naomi S. Hachiya, Kiyotoshi Kaneko

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

By utilizing a novel combinatorial method of a Laser Microdissection System and Western blot analysis, we demonstrate that a distinct isoform of abnormally phosphorylated tau (69 kDa, Tau 69) predominantly aggregated in laser-microdissected Pick bodies (PBs) in sporadic Pick's disease. By contrast, tau migrated as two major bands of 60 and 64 kDa (Tau 60 and 64) in total brain homogenates as previously reported. Comparative immunohistochemical analysis with anti-4-repeat antibody revealed that a major component of the abnormally phosphorylated tau in these PBs was 3-repeat tau (3R-tau). Whether 29 amino acid repeat encoded by exons 2 and 3 in the Tau 69 might accelerate the formation of PBs remains to be further investigated. Such a combination of morphological and biochemical techniques significantly complements the existing histopathological methods.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalAmyloid
Volume13
Issue number1
DOIs
Publication statusPublished - 2006 Mar

Keywords

  • Laser Microdissection System
  • Phosphorylated tau
  • Pick body
  • Pick's disease
  • Tau 60
  • Tau 64
  • Tau 69

ASJC Scopus subject areas

  • Internal Medicine

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