Three Distinct Southern Blot Hybridization Patterns of HBV-DNA in the Sera of HBV Carriers

Miki Yamada, Motoyasu Ishii, Masahito Miura, Shuichi Sato, Atsushi Kanno, Hitoshi Ohori, Takayoshi Toyota

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The molecular species of hepatitis B virus (HBV)-DNA of 44 sera taken from 25 HBV carriers were examined by Southern blot hybridization with a biotin-labeled HBV-DNA probe and classified into three patterns. Type I consisted of two distinct bands of 4.0 kb and 3.2 kb. Type II consisted of the 4.0 kb and 3.2 kb bands and a smear between these two bands. Type III showed a broad band below 4.0 kb. With endogenous HBV-DNA polymerase reaction, the smear disappeared and 4.0 kb and 3.2 kb bands appeared. After EcoRI digestion, the 4.0 kb band disappeared to form a single band of 3.2 kb. These results suggest that the 4.0 kb and 3.2 kb forms are a relaxed circular, fully double-stranded DNA and a linear, fully double-stranded DNA, respectively, and that the smear between 4.0 kb and 3.2 kb is formed from the sum of relaxed circular, partially double-stranded DNAs with various length of plus strands. Comparison between histological diagnosis and Southern blot hybridization patterns of 25 HBV carriers indicates that these three patterns are closely related to the degree of hepatitis.

Original languageEnglish
Pages (from-to)219-228
Number of pages10
JournalTohoku Journal of Experimental Medicine
Volume170
Issue number4
DOIs
Publication statusPublished - 1993

Keywords

  • HBV carriers
  • HBV-DNA
  • Southern blot hybridization

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Three Distinct Southern Blot Hybridization Patterns of HBV-DNA in the Sera of HBV Carriers'. Together they form a unique fingerprint.

Cite this