Three-dimensional tracking of quantum dot-conjugated molecules in living cells

Lucia Gardini, Martino Calamai, Hiroyasu Hatakeyama, Makoto Kanzaki, Marco Capitanio, Francesco Saverio Pavone

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

Here, we describe protocols for three-dimensional tracking of single quantum dot-conjugated molecules with nanometer accuracy in living cells using conventional fluorescence microscopy. The technique exploits out-of-focus images of single emitters combined with an automated pattern-recognition open-source software that fits the images with proper model functions to extract the emitter coordinates. We describe protocols for targeting quantum dots to both membrane components and cytosolic proteins.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages425-448
Number of pages24
DOIs
Publication statusPublished - 2018 Jan 1

Publication series

NameMethods in Molecular Biology
Volume1814
ISSN (Print)1064-3745

Keywords

  • Cytosolic proteins
  • Lipids
  • Living cells
  • Out-of-focus imaging
  • Plasma membrane proteins
  • Quantum dots
  • Single-molecule localization
  • Three-dimensional tracking

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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