Three-dimensional tracking of quantum dot-conjugated molecules in living cells

Lucia Gardini; Martino Calamai; Hiroyasu Hatakeyama; Makoto Kanzaki; Marco Capitanio; Francesco Saverio Pavone

doi:10.1007/978-1-4939-8591-3_26

Three-dimensional tracking of quantum dot-conjugated molecules in living cells

Lucia Gardini, Martino Calamai, Hiroyasu Hatakeyama, Makoto Kanzaki, Marco Capitanio, Francesco Saverio Pavone

Research output: Chapter in Book/Report/Conference proceeding › Chapter

5 Citations (Scopus)

Abstract

Here, we describe protocols for three-dimensional tracking of single quantum dot-conjugated molecules with nanometer accuracy in living cells using conventional fluorescence microscopy. The technique exploits out-of-focus images of single emitters combined with an automated pattern-recognition open-source software that fits the images with proper model functions to extract the emitter coordinates. We describe protocols for targeting quantum dots to both membrane components and cytosolic proteins.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	425-448
Number of pages	24
DOIs	https://doi.org/10.1007/978-1-4939-8591-3_26
Publication status	Published - 2018 Jan 1

Publication series

Name	Methods in Molecular Biology
Volume	1814
ISSN (Print)	1064-3745

Keywords

Cytosolic proteins
Lipids
Living cells
Out-of-focus imaging
Plasma membrane proteins
Quantum dots
Single-molecule localization
Three-dimensional tracking

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-8591-3_26

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Three-dimensional tracking of quantum dot-conjugated molecules in living cells. / Gardini, Lucia; Calamai, Martino; Hatakeyama, Hiroyasu; Kanzaki, Makoto; Capitanio, Marco; Pavone, Francesco Saverio.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 425-448 (Methods in Molecular Biology; Vol. 1814).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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