@inbook{67bf28e784d74cb28f199ad4abec62ea,
title = "Three-dimensional tracking of quantum dot-conjugated molecules in living cells",
abstract = "Here, we describe protocols for three-dimensional tracking of single quantum dot-conjugated molecules with nanometer accuracy in living cells using conventional fluorescence microscopy. The technique exploits out-of-focus images of single emitters combined with an automated pattern-recognition open-source software that fits the images with proper model functions to extract the emitter coordinates. We describe protocols for targeting quantum dots to both membrane components and cytosolic proteins.",
keywords = "Cytosolic proteins, Lipids, Living cells, Out-of-focus imaging, Plasma membrane proteins, Quantum dots, Single-molecule localization, Three-dimensional tracking",
author = "Lucia Gardini and Martino Calamai and Hiroyasu Hatakeyama and Makoto Kanzaki and Marco Capitanio and Pavone, {Francesco Saverio}",
note = "Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2018",
doi = "10.1007/978-1-4939-8591-3_26",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "425--448",
booktitle = "Methods in Molecular Biology",
}