Three dimensional structure observation of giant actin complexes by transmission electron microtomography

Kazuhiro Shikinaka, Hyuckjoon Kwon, Akira Kakugo, Hidemitsu Furukawa, Jian Ping Gong, Yoshihito Osada, Hideo Nishioka, Hiroshi Jinnai

Research output: Contribution to conferencePaperpeer-review

Abstract

We have reported that polymer-actin complexes are obtained by mixing F-actin with synthetic polymers carrying positive charges. We found that the polymer-actin complex shows various morphology depending on polycation and salt concentration. In this study, we report that the three dimensional structure analysis of polymer-actin complexes by using transmission electron microtomography (TEMT). We found that the polymer-actin complex is flattened bundle in the low salt concentration, while that is cylindrical bundle in high salt concentration. It means that three dimensional structure of polymer-actin complex is determined by the magnitude of electrostatic repulsion force in F-actins.

Original languageEnglish
Pages3921-3922
Number of pages2
Publication statusPublished - 2006 Dec 1
Externally publishedYes
Event55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan
Duration: 2006 Sep 202006 Sep 22

Other

Other55th Society of Polymer Science Japan Symposium on Macromolecules
CountryJapan
CityToyama
Period06/9/2006/9/22

Keywords

  • Actin
  • Polycation
  • Polyion complex
  • Transmission electron microtomography

ASJC Scopus subject areas

  • Engineering(all)

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