Three dimensional structure observation of giant actin complexes by transmission electron microtomography

Kazuhiro Shikinaka; Hyuckjoon Kwon; Akira Kakugo; Hidemitsu Furukawa; Jian Ping Gong; Yoshihito Osada; Hideo Nishioka; Hiroshi Jinnai

Three dimensional structure observation of giant actin complexes by transmission electron microtomography

Kazuhiro Shikinaka, Hyuckjoon Kwon, Akira Kakugo, Hidemitsu Furukawa, Jian Ping Gong, Yoshihito Osada, Hideo Nishioka, Hiroshi Jinnai

Research output: Contribution to conference › Paper › peer-review

Abstract

We have reported that polymer-actin complexes are obtained by mixing F-actin with synthetic polymers carrying positive charges. We found that the polymer-actin complex shows various morphology depending on polycation and salt concentration. In this study, we report that the three dimensional structure analysis of polymer-actin complexes by using transmission electron microtomography (TEMT). We found that the polymer-actin complex is flattened bundle in the low salt concentration, while that is cylindrical bundle in high salt concentration. It means that three dimensional structure of polymer-actin complex is determined by the magnitude of electrostatic repulsion force in F-actins.

Original language	English
Pages	3921-3922
Number of pages	2
Publication status	Published - 2006 Dec 1
Externally published	Yes
Event	55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan Duration: 2006 Sep 20 → 2006 Sep 22

Other

Other	55th Society of Polymer Science Japan Symposium on Macromolecules
Country/Territory	Japan
City	Toyama
Period	06/9/20 → 06/9/22

Keywords

Actin
Polycation
Polyion complex
Transmission electron microtomography

ASJC Scopus subject areas

Engineering(all)

Cite this

@conference{d968343dd6cd4abb99c4ac5708abf3fe,

title = "Three dimensional structure observation of giant actin complexes by transmission electron microtomography",

abstract = "We have reported that polymer-actin complexes are obtained by mixing F-actin with synthetic polymers carrying positive charges. We found that the polymer-actin complex shows various morphology depending on polycation and salt concentration. In this study, we report that the three dimensional structure analysis of polymer-actin complexes by using transmission electron microtomography (TEMT). We found that the polymer-actin complex is flattened bundle in the low salt concentration, while that is cylindrical bundle in high salt concentration. It means that three dimensional structure of polymer-actin complex is determined by the magnitude of electrostatic repulsion force in F-actins.",

keywords = "Actin, Polycation, Polyion complex, Transmission electron microtomography",

author = "Kazuhiro Shikinaka and Hyuckjoon Kwon and Akira Kakugo and Hidemitsu Furukawa and Gong, {Jian Ping} and Yoshihito Osada and Hideo Nishioka and Hiroshi Jinnai",

year = "2006",

month = dec,

day = "1",

language = "English",

pages = "3921--3922",

note = "55th Society of Polymer Science Japan Symposium on Macromolecules ; Conference date: 20-09-2006 Through 22-09-2006",

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TY - CONF

T1 - Three dimensional structure observation of giant actin complexes by transmission electron microtomography

AU - Shikinaka, Kazuhiro

AU - Kwon, Hyuckjoon

AU - Kakugo, Akira

AU - Furukawa, Hidemitsu

AU - Gong, Jian Ping

AU - Osada, Yoshihito

AU - Nishioka, Hideo

AU - Jinnai, Hiroshi

PY - 2006/12/1

Y1 - 2006/12/1

N2 - We have reported that polymer-actin complexes are obtained by mixing F-actin with synthetic polymers carrying positive charges. We found that the polymer-actin complex shows various morphology depending on polycation and salt concentration. In this study, we report that the three dimensional structure analysis of polymer-actin complexes by using transmission electron microtomography (TEMT). We found that the polymer-actin complex is flattened bundle in the low salt concentration, while that is cylindrical bundle in high salt concentration. It means that three dimensional structure of polymer-actin complex is determined by the magnitude of electrostatic repulsion force in F-actins.

AB - We have reported that polymer-actin complexes are obtained by mixing F-actin with synthetic polymers carrying positive charges. We found that the polymer-actin complex shows various morphology depending on polycation and salt concentration. In this study, we report that the three dimensional structure analysis of polymer-actin complexes by using transmission electron microtomography (TEMT). We found that the polymer-actin complex is flattened bundle in the low salt concentration, while that is cylindrical bundle in high salt concentration. It means that three dimensional structure of polymer-actin complex is determined by the magnitude of electrostatic repulsion force in F-actins.

KW - Actin

KW - Polycation

KW - Polyion complex

KW - Transmission electron microtomography

UR - http://www.scopus.com/inward/record.url?scp=33846129097&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846129097&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33846129097

SP - 3921

EP - 3922

T2 - 55th Society of Polymer Science Japan Symposium on Macromolecules

Y2 - 20 September 2006 through 22 September 2006

ER -

Three dimensional structure observation of giant actin complexes by transmission electron microtomography

Abstract

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Keywords

ASJC Scopus subject areas

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