Three-dimensional micro-culture system with a silicon-based cell array device for multi-channel drug sensitivity test

Yu Suke Torisawa, Hitoshi Shiku, Tomoyuki Yasukawa, Matsuhiko Nishizawa, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

The oxygen consumption rate of three-dimensional cultured cells integrated in a silicon chip was non-invasively quantified by scanning electrochemical microscopy (SECM). The cells were embedded in a collagen gel matrix and entrapped in silicon microstructures. The oxygen concentration profile near the cell panel agreed well with the theoretically determined spherical diffusion. The oxygen consumption rate (F) of a single cell was calculated as (1.0 ± 0.19) × 10-16 mol s-1. The oxygen consumption of a cell panel increased along with the cellular proliferation; the increase in respiration activity was in accordance with the increase in cell numbers. Our three-dimensional micro-culture system combined with SECM enables continuous quantification of the oxygen consumption rate using very small amounts of sample.

Original languageEnglish
Pages (from-to)654-659
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume108
Issue number1-2 SPEC. ISS.
DOIs
Publication statusPublished - 2005 Jul 22

Keywords

  • Cell chip
  • Collagen gel
  • Scanning electrochemical microscopy
  • Three-dimensional cell culture

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Fingerprint Dive into the research topics of 'Three-dimensional micro-culture system with a silicon-based cell array device for multi-channel drug sensitivity test'. Together they form a unique fingerprint.

Cite this