Three-dimensional laser-scanning confocal reflecting microscope for multicolor single-molecule imaging at 1.5 K

Minako Maruo, Hironori Inagawa, Yasuharu Toratani, Toru Kondo, Michio Matsushita, Satoru Fujiyoshi

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

A three-dimensional (3D) laser-scanning confocal microscope was developed for multicolor 3D co-localization of single fluorescent molecules immobilized at a temperature of 1.5 K. The image quality was tested by taking 3D emission image of single quantum dots with three different excitation wavelengths of 635, 532 and 405 nm. The 3D image size was all within 1.2 times of the diffraction-limited size. From the centroid position of the 3D image taken with 635- and 532-nm excitations, 3D co-localization precision of single dot was determined to be 3 nm in the lateral (x, y) and 18 nm in the axial (z) directions.

Original languageEnglish
Pages (from-to)233-236
Number of pages4
JournalChemical Physics Letters
Volume591
DOIs
Publication statusPublished - 2014 Jan 20
Externally publishedYes

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

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