TY - JOUR
T1 - Three-dimensional laser-scanning confocal reflecting microscope for multicolor single-molecule imaging at 1.5 K
AU - Maruo, Minako
AU - Inagawa, Hironori
AU - Toratani, Yasuharu
AU - Kondo, Toru
AU - Matsushita, Michio
AU - Fujiyoshi, Satoru
N1 - Funding Information:
This Letter was financially supported by Grants-in-Aid for Scientific Research ( 23685002 and 23350004 ) Ministry of Education, Culture, Sports, Science and Technology of Japan, and by Yamada Science Foundation.
PY - 2014/1/20
Y1 - 2014/1/20
N2 - A three-dimensional (3D) laser-scanning confocal microscope was developed for multicolor 3D co-localization of single fluorescent molecules immobilized at a temperature of 1.5 K. The image quality was tested by taking 3D emission image of single quantum dots with three different excitation wavelengths of 635, 532 and 405 nm. The 3D image size was all within 1.2 times of the diffraction-limited size. From the centroid position of the 3D image taken with 635- and 532-nm excitations, 3D co-localization precision of single dot was determined to be 3 nm in the lateral (x, y) and 18 nm in the axial (z) directions.
AB - A three-dimensional (3D) laser-scanning confocal microscope was developed for multicolor 3D co-localization of single fluorescent molecules immobilized at a temperature of 1.5 K. The image quality was tested by taking 3D emission image of single quantum dots with three different excitation wavelengths of 635, 532 and 405 nm. The 3D image size was all within 1.2 times of the diffraction-limited size. From the centroid position of the 3D image taken with 635- and 532-nm excitations, 3D co-localization precision of single dot was determined to be 3 nm in the lateral (x, y) and 18 nm in the axial (z) directions.
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U2 - 10.1016/j.cplett.2013.11.039
DO - 10.1016/j.cplett.2013.11.039
M3 - Article
AN - SCOPUS:84890288755
VL - 591
SP - 233
EP - 236
JO - Chemical Physics Letters
JF - Chemical Physics Letters
SN - 0009-2614
ER -