Three-dimensional imaging of the vasculature in chicken embryo by combination of ultrasonic and photoacoustic imaging

Mika Sato, Takuya Izumi, Yoshifumi Saijo, Yuji Watanabe, Harukazu Nakamura

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Vascular network is a good target for PA imaging with conventional 532 nm laser because the extinction coefficient is of red blood cell is high between 500 and 600 nm. Compared with US Doppler technique, PA imaging is superior for visualization of low speed blood flow in small vessels. Laser pulses were generated by a semiconductor diode laser with the wavelength of 532 nm, the pulse width of 3.4 ns. PA signal was received by a 50 MHz concave ultrasound transducer with a 1 mm hole in the central part to get through an optical fiber in order to concentrically align the light illumination and signal reception. Fertile chicken eggs were placed in a shallow dish after 3 days of incubation for easy observation with US and PA imaging. US imaging visualized overview of the whole body, especially, the vertebral column were clearly displayed. PA imaging visualized the heart and great vessels including aortic arch. This study showed the feasibility of US-PA combined imaging for observation of ex ovo chicken embryo and future application of the imaging modality in the developmental biology.

Original languageEnglish
Title of host publication2013 IEEE International Ultrasonics Symposium, IUS 2013
Pages1532-1533
Number of pages2
DOIs
Publication statusPublished - 2013 Dec 1
Event2013 IEEE International Ultrasonics Symposium, IUS 2013 - Prague, Czech Republic
Duration: 2013 Jul 212013 Jul 25

Publication series

NameIEEE International Ultrasonics Symposium, IUS
ISSN (Print)1948-5719
ISSN (Electronic)1948-5727

Other

Other2013 IEEE International Ultrasonics Symposium, IUS 2013
CountryCzech Republic
CityPrague
Period13/7/2113/7/25

Keywords

  • Chicken embryo
  • Developmental biology
  • High frequency ultrasound
  • Photoacoustic imaging
  • Three-dimensional

ASJC Scopus subject areas

  • Acoustics and Ultrasonics

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  • Cite this

    Sato, M., Izumi, T., Saijo, Y., Watanabe, Y., & Nakamura, H. (2013). Three-dimensional imaging of the vasculature in chicken embryo by combination of ultrasonic and photoacoustic imaging. In 2013 IEEE International Ultrasonics Symposium, IUS 2013 (pp. 1532-1533). [6724999] (IEEE International Ultrasonics Symposium, IUS). https://doi.org/10.1109/ULTSYM.2013.0389