Three-dimensional fine structure of feeder organelle in Cryptosporidium parvum

Hiroki Bochimoto, Daisuke Kondoh, Yo Ishihara, Mohammad Hazzaz Bin Kabir, Kentaro Kato

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Feeder organelles of Cryptosporidium are the convoluted structures located at the host-parasite interface that uptake of nutrients from host cells. Although the ultrastructure of feeder organelles has been summarized as being highly invaginated structure, the three-dimensional form remains uncertain. Osmium-maceration scanning electron microscopy (OS-SEM) allows visualization of the three-dimensional ultrastructure after removing soluble proteins. Here, we assessed C. parvum attached to mouse ileal epithelial cells using transmission electron microscopy (TEM) and OS-SEM. Feeder organelles visualized by TEM as aggregated structures of concentrically-, vertically- and randomly-lined bars comprised a complex reticulated network of stacked flat bursiform, ring-shaped bursiform and reticulated tubular membranes on OS-SEM. These findings suggested that the feeder organelles are more complex than was previously thought.

Original languageEnglish
Article number101958
JournalParasitology International
Volume73
DOIs
Publication statusPublished - 2019 Dec

Keywords

  • Cryptosporidium
  • Feeder organelle
  • Osmium maceration
  • SEM
  • TEM

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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