Three-dimensional fine structure of feeder organelle in Cryptosporidium parvum

Hiroki Bochimoto, Daisuke Kondoh, Yo Ishihara, Mohammad Hazzaz Bin Kabir, Kentaro Kato

    Research output: Contribution to journalArticlepeer-review

    1 Citation (Scopus)

    Abstract

    Feeder organelles of Cryptosporidium are the convoluted structures located at the host-parasite interface that uptake of nutrients from host cells. Although the ultrastructure of feeder organelles has been summarized as being highly invaginated structure, the three-dimensional form remains uncertain. Osmium-maceration scanning electron microscopy (OS-SEM) allows visualization of the three-dimensional ultrastructure after removing soluble proteins. Here, we assessed C. parvum attached to mouse ileal epithelial cells using transmission electron microscopy (TEM) and OS-SEM. Feeder organelles visualized by TEM as aggregated structures of concentrically-, vertically- and randomly-lined bars comprised a complex reticulated network of stacked flat bursiform, ring-shaped bursiform and reticulated tubular membranes on OS-SEM. These findings suggested that the feeder organelles are more complex than was previously thought.

    Original languageEnglish
    Article number101958
    JournalParasitology International
    Volume73
    DOIs
    Publication statusPublished - 2019 Dec

    Keywords

    • Cryptosporidium
    • Feeder organelle
    • Osmium maceration
    • SEM
    • TEM

    ASJC Scopus subject areas

    • Parasitology
    • Infectious Diseases

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