Thr80 of Sheep Podoplanin Is a Critical Epitope of the Antisheep Podoplanin Monoclonal Antibody: PMab-256

Yukinari Kato, Masato Sano, Teizo Asano, Yusuke Sayama, Mika K. Kaneko

Research output: Contribution to journalArticlepeer-review

Abstract

An antisheep podoplanin (sPDPN) monoclonal antibody (mAb), PMab-256, has recently been established. PMab-256 shows positive immunostaining for lymphatic endothelial cells, lung type I alveolar cells, and kidney podocytes. PDPN possesses three platelet-aggregation-stimulating (PLAG) domains, PLAG1, PLAG2, and PLAG3, and a PLAG-like domain (PLD). The binding epitope of many anti-PDPN mAbs is located in PLAG domains or PLD. The purpose of this study is to determine the binding epitope of PMab-256. Analysis of sPDPN deletion mutants revealed that the N-terminus of the PMab-256 epitope exists between amino acids 75 and 80 of sPDPN. Furthermore, analysis of sPDPN point mutations demonstrated that the critical epitope includes Thr80 of sPDPN, indicating that the PMab-256 epitope is in the PLD of sPDPN.

Original languageEnglish
Pages (from-to)95-100
Number of pages6
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Volume39
Issue number3
DOIs
Publication statusPublished - 2020 Jun

Keywords

  • PMab-256
  • epitope
  • monoclonal antibody
  • sheep podoplanin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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