Thirteen novel deoxynivalenol-degrading bacteria are classified within two genera with distinct degradation mechanisms

Ikuo Sato, Michihiro Ito, Masumi Ishizaka, Yoko Ikunaga, Yukari Sato, Shigenobu Yoshida, Motoo Koitabashi, Seiya Tsushima

Research output: Contribution to journalArticlepeer-review

58 Citations (Scopus)

Abstract

The mycotoxin deoxynivalenol (DON), a secondary metabolite produced by species of the plant pathogen Fusarium, causes serious problems in cereal crop production because of its toxicity towards humans and livestock. A biological approach for the degradation of DON using a DON-degrading bacterium (DDB) appears to be promising, although information about DDBs is limited. We isolated 13 aerobic DDBs from a variety of environmental samples, including field soils and wheat leaves. Of these 13 strains, nine belonged to the Gram-positive genus Nocardioides and other four to the Gram-negative genus Devosia. The degradation phenotypes of the two Gram types were clearly different; all washed cells of the 13 strains degraded 100 μg mL -1 DON to below the detection limit (0.5 μg mL -1), but the conditions inducing the DON-degrading activities differed between the two Gram types. The HPLC profiles of the DON metabolites were also distinct between the two genera, although all strains produced 3-epi-deoxynivalenol. The Gram-positive strains showed DON assimilation in media containing DON as a carbon source, whereas the Gram-negatives did not. Our results suggest that aerobic DDBs are distributed within at least two phylogenetically restricted genera, suggesting independent evolution of the DON-degradation mechanisms.

Original languageEnglish
Pages (from-to)110-117
Number of pages8
JournalFEMS Microbiology Letters
Volume327
Issue number2
DOIs
Publication statusPublished - 2012 Feb

Keywords

  • Devosia
  • Fsarium mycotoxin
  • Mycotoxin degradation
  • Nocardioides
  • Trichothecenes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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