Thioredoxin-1 Attenuates Early Graft Loss after Intraportal Islet Transplantation in Mice

Kengo Asami, Akiko Inagaki, Takehiro Imura, Satoshi Sekiguchi, Keisei Fujimori, Hiroshi Masutani, Junji Yodoi, Susumu Satomi, Noriaki Ohuchi, Masafumi Goto

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)


Aims:Recent studies suggest that decreasing oxidative stress is crucial to achieve successful islet transplantation. Thioredoxin-1 (TRX), which is a multifunctional redox-active protein, has been reported to suppress oxidative stress. Furthermore, it also has anti-inflammatory and anti-apoptotic effects. In this study, we investigated the effects of TRX on early graft loss after islet transplantation.Methods:Intraportal islet transplantation was performed for two groups of streptozotocin-induced diabetic mice: a control and a TRX group. In addition, TRX-transgenic (Tg) mice were alternately used as islet donors or recipients.Results:The changes in blood glucose levels were significantly lower in the TRX group compared with the TRX-Tg donor and control groups (p<0.01). Glucose tolerance and the residual graft mass were considerably better in the TRX group. TRX significantly suppressed the serum levels of interleukin-1β (p<0.05), although neither anti-apoptotic nor anti-chemotactic effects were observed. Notably, no increase in the 8-hydroxy-2′-deoxyguanosine level was observed after islet infusion, irrespective of TRX administration.Conclusions:The present study demonstrates that overexpression of TRX on the islet grafts is not sufficient to improve engraftment. In contrast, TRX administration to the recipients exerts protective effects on transplanted islet grafts by suppressing the serum levels of interleukin-1β. However, TRX alone appears to be insufficient to completely prevent early graft loss after islet transplantation. We therefore propose that a combination of TRX and other anti-inflammatory treatments represents a promising regimen for improving the efficacy of islet transplantation.

Original languageEnglish
Article numbere70259
JournalPloS one
Issue number8
Publication statusPublished - 2013 Aug 9

ASJC Scopus subject areas

  • General


Dive into the research topics of 'Thioredoxin-1 Attenuates Early Graft Loss after Intraportal Islet Transplantation in Mice'. Together they form a unique fingerprint.

Cite this