TY - JOUR
T1 - Thermostability of tropomyosins from the fast skeletal muscles of tropical fish species
AU - Huang, Ming Chih
AU - Lee, Cheng Linn
AU - Ochiai, Yoshihiro
AU - Watabe, Shugo
PY - 2019/6/15
Y1 - 2019/6/15
N2 - In order to investigate the species-specific heat tolerance of tropical fishes, the thermodynamic properties of muscle tropomyosin, a member of myofibrillar proteins, were compared among milkfish, tilapia, grouper, and mudskipper. The purified tropomyosins were subjected to differential scanning calorimetry and circular dichroism spectrometry. To unveil the relationship between the stability and the amino acid sequences, the muscle tropomyosin genes of the four species were also cloned, and their deduced amino acid sequences were compared. Thermodynamic analysis revealed that the milkfish tropomyosin showed lower refolding ability after thermal denaturation, compared with those of the other species. The amino acid sequences of these tropomyosins were similar to each other, with the identity being in the range of 95–96%.
AB - In order to investigate the species-specific heat tolerance of tropical fishes, the thermodynamic properties of muscle tropomyosin, a member of myofibrillar proteins, were compared among milkfish, tilapia, grouper, and mudskipper. The purified tropomyosins were subjected to differential scanning calorimetry and circular dichroism spectrometry. To unveil the relationship between the stability and the amino acid sequences, the muscle tropomyosin genes of the four species were also cloned, and their deduced amino acid sequences were compared. Thermodynamic analysis revealed that the milkfish tropomyosin showed lower refolding ability after thermal denaturation, compared with those of the other species. The amino acid sequences of these tropomyosins were similar to each other, with the identity being in the range of 95–96%.
KW - Amino acid sequence
KW - Muscle tropomyosin
KW - Thermostability
KW - Tropical fish
UR - http://www.scopus.com/inward/record.url?scp=85064334168&partnerID=8YFLogxK
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U2 - 10.1007/s10695-019-00632-7
DO - 10.1007/s10695-019-00632-7
M3 - Article
C2 - 30945041
AN - SCOPUS:85064334168
VL - 45
SP - 1189
EP - 1202
JO - Fish Physiology and Biochemistry
JF - Fish Physiology and Biochemistry
SN - 0920-1742
IS - 3
ER -