Abstract
A triple-helix-forming collagen model peptide, (prolyl-trans-4-hydroxyprolyl-glycyl)10 [(Pro-Hyp-Gly)10], and a thermosensitive elastin-derived pentapeptide, valyl-prolyl-glycyl-valyl-glycyl (Val-Pro-Gly-Val-Gly), were copolymerized in various mole ratios using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride and 1-hydroxy-benzotriazole in dimethyl sulfoxide at 20 °C. All of the obtained polypeptides have molecular weight higher than 10 3 and contain a triple-helical structure, and showed an inverse phase transition from transparent solution to turbid suspension in response to a rise in temperature. The lower critical solution temperature of the polypeptide solution decreased upon increasing the content of Val-Pro-Gly-Val-Gly. Furthermore, polypeptides containing 82-86 mol % of Val-Pro-Gly-Val-Gly in composition showed reversible gel formation, suggesting that (Pro-Hyp-Gly)10 acts as a hydrated unit and Val-Pro-Gly-Val-Gly acts as a thermosensitive crosslinking point. These biodegradable thermosensitive polypeptides may be useful for biomedical applications, including, as a scaffold for tissue regeneration.
Original language | English |
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Pages (from-to) | 6048-6056 |
Number of pages | 9 |
Journal | Journal of Polymer Science, Part A: Polymer Chemistry |
Volume | 43 |
Issue number | 23 |
DOIs | |
Publication status | Published - 2005 Dec 1 |
Externally published | Yes |
Keywords
- Collagen-like polypeptides
- Elastin-derived peptide
- Lower critical solution temperature
- Thermosensitive sol-gel transition
ASJC Scopus subject areas
- Polymers and Plastics
- Organic Chemistry
- Materials Chemistry