Thermosensitive gel formation of novel polypeptides containing a collagen-derived Pro-Hyp-Gly sequence and an elastin-derived Val-Pro-Gly-Val-Gly sequence

Yasushi Morihara, Shin Ichi Ogata, Masanobu Kamitakahara, Chikara Ohtsuki, Masao Tanihara

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

A triple-helix-forming collagen model peptide, (prolyl-trans-4-hydroxyprolyl-glycyl)10 [(Pro-Hyp-Gly)10], and a thermosensitive elastin-derived pentapeptide, valyl-prolyl-glycyl-valyl-glycyl (Val-Pro-Gly-Val-Gly), were copolymerized in various mole ratios using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride and 1-hydroxy-benzotriazole in dimethyl sulfoxide at 20 °C. All of the obtained polypeptides have molecular weight higher than 10 3 and contain a triple-helical structure, and showed an inverse phase transition from transparent solution to turbid suspension in response to a rise in temperature. The lower critical solution temperature of the polypeptide solution decreased upon increasing the content of Val-Pro-Gly-Val-Gly. Furthermore, polypeptides containing 82-86 mol % of Val-Pro-Gly-Val-Gly in composition showed reversible gel formation, suggesting that (Pro-Hyp-Gly)10 acts as a hydrated unit and Val-Pro-Gly-Val-Gly acts as a thermosensitive crosslinking point. These biodegradable thermosensitive polypeptides may be useful for biomedical applications, including, as a scaffold for tissue regeneration.

Original languageEnglish
Pages (from-to)6048-6056
Number of pages9
JournalJournal of Polymer Science, Part A: Polymer Chemistry
Volume43
Issue number23
DOIs
Publication statusPublished - 2005 Dec 1
Externally publishedYes

Keywords

  • Collagen-like polypeptides
  • Elastin-derived peptide
  • Lower critical solution temperature
  • Thermosensitive sol-gel transition

ASJC Scopus subject areas

  • Polymers and Plastics
  • Organic Chemistry
  • Materials Chemistry

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