Thermophilic fermentative hydrogen production from starch-wastewater with bio-granules

Yohei Akutsu, Dong Yeol Lee, Yong Zhi Chi, Gyokuyu Ri, Hideki Harada, Han Qing Yu

Research output: Contribution to journalArticlepeer-review

59 Citations (Scopus)


In this study, the effects of the hydraulic retention time (HRT), pH and substrate concentration on the thermophilic hydrogen production of starch with an upflow anaerobic sludge bed (UASB) reactor were investigated. Starch was used as a sole substrate. Continuous hydrogen production was stably attained with a maximum H2 yield of 1.7 mol H2/mol glucose. A H2-producing thermophilic granule was successfully formed with diameter in the range of 0.5-4.0 mm with thermally pretreated methanogenic granules as the nuclei. The metabolic pathway of the granules was drastically changed at each operational parameter. The production of formic or lactic acids is an indication of the deterioration of hydrogen production for H2-producing thermophilic granular sludge.

Original languageEnglish
Pages (from-to)5061-5071
Number of pages11
JournalInternational Journal of Hydrogen Energy
Issue number12
Publication statusPublished - 2009 Jun 1


  • Bio-hydrogen
  • Granule
  • Starch-wastewater
  • Thermophilic
  • UASB reactor

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology


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