Thermogemmatispora aurantia sp. nov. and thermogemmatispora argillosa sp. nov., within the class ktedonobacteria, and emended description of the genus thermogemmatispora

Yu Zheng, Chiung Mei Wang, Yasuteru Sakai, Keietsu Abe, Akira Yokota, Shuhei Yabe

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Two thermophilic, aerobic, Gram-stain-positive Ktedonobacteria strains, A1-2T and A3-2T, were isolated from geothermal soil in Japan. The strains formed orange-coloured colonies on 10-fold diluted Reasoner’s 2A medium, followed by formation of branched aerial mycelium with multiple grape-like spores. Both strains hydrolysed casein, carboxymethyl cellulose, starch, chitin and xylan, but did not liquify gelatin. Strain A1-2T utilised sucrose and gellan gum and was inhibited by inositol, while strain A3-2T utilised only gellan gum and was not inhibited by inositol. The DNA G+C contents of strain A1-2T and A3-2T were 63.2 and 63.1 mol%, respectively. Chemotaxonomic data (major fatty acid, iso-C17: 0; major menaquinone, MK-9(H2); cell-wall amino acids, ornithine, serine, glycine, glutamic acid, alanine and b-alanine; polar lipids, phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol, one unidentified lipid, one unidentified phosphoglycolipid and three unidentified glycolipids; major cell-wall sugars, mannose, arabinose and xylose) indicate that both strains belong to the genus Thermogemmatispora. 16S rRNA gene sequence analysis indicated that strain A1-2T was most closely related to the type strains of Thermogemmatispora onikobensis (97.7% sequence similarity), and that strain A3-2T was most closely related to the type strains of Thermogemmatispora carboxidivorans(97.2%), but DNA–DNA hybridization shows relatedness values of <67% with previously described type strains. Moreover, 16S rRNA gene sequence similarity and DNA–DNA relatedness between strain A1-2T and strain A3-2T were 96.0 and 33.4%, respectively, suggesting that the two strains are genetically distinct. The two strains are proposed as Thermogemmatispora aurantia sp. nov. and Thermogemmatispora argillosa sp. nov.

Original languageEnglish
Article number003388
Pages (from-to)1744-1750
Number of pages7
JournalInternational journal of systematic and evolutionary microbiology
Volume69
Issue number6
DOIs
Publication statusPublished - 2019 Jun

Keywords

  • Genus Thermogemmatispora
  • Ktedonobacteria

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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