TY - JOUR
T1 - Thermogemmatispora aurantia sp. nov. and thermogemmatispora argillosa sp. nov., within the class ktedonobacteria, and emended description of the genus thermogemmatispora
AU - Zheng, Yu
AU - Wang, Chiung Mei
AU - Sakai, Yasuteru
AU - Abe, Keietsu
AU - Yokota, Akira
AU - Yabe, Shuhei
N1 - Funding Information:
This study was supported in part by MEXT/JSPS KAKENHI (Grant Number: 18K05406 and 16H06279), the Institute for Fermentation, Osaka (grant no. G-2018-1-038), and the China Scholarship Council (CSC number: 201606330094).
Publisher Copyright:
© 2019 IUMS.
PY - 2019/6
Y1 - 2019/6
N2 - Two thermophilic, aerobic, Gram-stain-positive Ktedonobacteria strains, A1-2T and A3-2T, were isolated from geothermal soil in Japan. The strains formed orange-coloured colonies on 10-fold diluted Reasoner’s 2A medium, followed by formation of branched aerial mycelium with multiple grape-like spores. Both strains hydrolysed casein, carboxymethyl cellulose, starch, chitin and xylan, but did not liquify gelatin. Strain A1-2T utilised sucrose and gellan gum and was inhibited by inositol, while strain A3-2T utilised only gellan gum and was not inhibited by inositol. The DNA G+C contents of strain A1-2T and A3-2T were 63.2 and 63.1 mol%, respectively. Chemotaxonomic data (major fatty acid, iso-C17: 0; major menaquinone, MK-9(H2); cell-wall amino acids, ornithine, serine, glycine, glutamic acid, alanine and b-alanine; polar lipids, phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol, one unidentified lipid, one unidentified phosphoglycolipid and three unidentified glycolipids; major cell-wall sugars, mannose, arabinose and xylose) indicate that both strains belong to the genus Thermogemmatispora. 16S rRNA gene sequence analysis indicated that strain A1-2T was most closely related to the type strains of Thermogemmatispora onikobensis (97.7% sequence similarity), and that strain A3-2T was most closely related to the type strains of Thermogemmatispora carboxidivorans(97.2%), but DNA–DNA hybridization shows relatedness values of <67% with previously described type strains. Moreover, 16S rRNA gene sequence similarity and DNA–DNA relatedness between strain A1-2T and strain A3-2T were 96.0 and 33.4%, respectively, suggesting that the two strains are genetically distinct. The two strains are proposed as Thermogemmatispora aurantia sp. nov. and Thermogemmatispora argillosa sp. nov.
AB - Two thermophilic, aerobic, Gram-stain-positive Ktedonobacteria strains, A1-2T and A3-2T, were isolated from geothermal soil in Japan. The strains formed orange-coloured colonies on 10-fold diluted Reasoner’s 2A medium, followed by formation of branched aerial mycelium with multiple grape-like spores. Both strains hydrolysed casein, carboxymethyl cellulose, starch, chitin and xylan, but did not liquify gelatin. Strain A1-2T utilised sucrose and gellan gum and was inhibited by inositol, while strain A3-2T utilised only gellan gum and was not inhibited by inositol. The DNA G+C contents of strain A1-2T and A3-2T were 63.2 and 63.1 mol%, respectively. Chemotaxonomic data (major fatty acid, iso-C17: 0; major menaquinone, MK-9(H2); cell-wall amino acids, ornithine, serine, glycine, glutamic acid, alanine and b-alanine; polar lipids, phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol, one unidentified lipid, one unidentified phosphoglycolipid and three unidentified glycolipids; major cell-wall sugars, mannose, arabinose and xylose) indicate that both strains belong to the genus Thermogemmatispora. 16S rRNA gene sequence analysis indicated that strain A1-2T was most closely related to the type strains of Thermogemmatispora onikobensis (97.7% sequence similarity), and that strain A3-2T was most closely related to the type strains of Thermogemmatispora carboxidivorans(97.2%), but DNA–DNA hybridization shows relatedness values of <67% with previously described type strains. Moreover, 16S rRNA gene sequence similarity and DNA–DNA relatedness between strain A1-2T and strain A3-2T were 96.0 and 33.4%, respectively, suggesting that the two strains are genetically distinct. The two strains are proposed as Thermogemmatispora aurantia sp. nov. and Thermogemmatispora argillosa sp. nov.
KW - Genus Thermogemmatispora
KW - Ktedonobacteria
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U2 - 10.1099/ijsem.0.003388
DO - 10.1099/ijsem.0.003388
M3 - Article
C2 - 30950784
AN - SCOPUS:85067087800
VL - 69
SP - 1744
EP - 1750
JO - International Journal of Systematic Bacteriology
JF - International Journal of Systematic Bacteriology
SN - 1466-5026
IS - 6
M1 - 003388
ER -