Thermal stability of the synthetic peptides with the sequence of fish fast skeletal muscle tropomyosin

Hideo Ozawa, Shugo Watabe, Yoshihiro Ochiai

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Tropomyosins from fish skeletal muscle show high amino acid sequence homology, although their thermal stability is clearly different among species. In order to determine the regions that are responsible for the stability of this protein, five synthetic peptides of 30mer were synthesized by Fmoc method, based on the sequence of walleye pollack Theragra chalcogramma fast skeletal muscle tropomyosin, namely, N terminal Met1-Lys30, the variable region Asp84-Leu113, the middle region Val 128-Ala157, the region containing the conservative Cys (Leu176-Lys205), and C terminal Asp255-Ile 284. The thermal stability of these peptides was measured by circular dichroism and differential scanning calorimetry. The helical contents of these peptides were decreased in a temperature-dependent manner, although they showed no clear melting temperature, suggesting that the enthalpy necessary for the complete denaturation of these peptides was low. Peptides Asp 255-Ile284 and Asp84-Leu113 showed the highest and second highest α-helical contents, respectively, and the other peptides gave rise to lower α-helical contents.

Original languageEnglish
Pages (from-to)1029-1037
Number of pages9
JournalFisheries Science
Volume75
Issue number4
DOIs
Publication statusPublished - 2009 Jul 1
Externally publishedYes

Keywords

  • Circular dichroism spectrum
  • Coiled-coil
  • Skeletal muscle
  • Synthetic peptide
  • Thermal stability
  • Tropomyosin
  • Walleye pollack
  • α-Helix

ASJC Scopus subject areas

  • Aquatic Science

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