Thermal resistance traits of the clonal silver crucian carp Carassius langsdorfii and evaluation of these traits using primary culture cells

Kenji Sakamoto, Worawut Koedprang, Masamichi Nakajima, Nobuhiko Taniguchi

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The thermal resistance traits of three clonal lines of the silver crucian carp Carassius langsdorfii (SCC-1, -2 and -3) were investigated. Individual juvenile fish reared at 20°C were exposed to thermal stress at 36.5±0.5°C and their death times during this exposure were measured. The death times of SCC-2 and SCC-3 fish were 66.6±31.2 and 144.7±49.8 min, respectively. In contrast, all SCC-1 fish survived under these conditions. Furthermore, the thermal sensitivity of primary culture cells from each clone was determined at 37, 40 or 43°C by the Trypan blue assay. Under all treatment conditions, the thermal sensitivity of the SCC-1 primary cultures was lower than those of the others. These results estimated the correlation between the in vivo and in vitro thermal resistances. Therefore, the use of primary culture cells to evaluate thermal resistance could be a useful method of selection breeding.

Original languageEnglish
Pages (from-to)1029-1033
Number of pages5
JournalFisheries Science
Volume68
Issue number5
DOIs
Publication statusPublished - 2002 Oct

Keywords

  • Carassius langsdorfii
  • Clonal line
  • Primary culture cell
  • Silver crucian carp
  • Thermal resistance

ASJC Scopus subject areas

  • Aquatic Science

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