Therapeutic effect of anti-TNF-α antibody and levofloxacin (LVFX) in a mouse model of enterohemorrhagic Escherichia coli 0157 infection

Emiko Isogai, Hiroshi Isogai, Kimiharu Hirose, Toru Kubota, Koichi Kimura, Nobuhiro Fujii, Shunji Hayashi, Koichi Takeshi, Keiji Oguma

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The ability of an anti-TNF-α antibody to confer protection against enterohaemorrhagic Escherichia coli (EHEC) O157 was investigated in germfree IQI mice. The use of an antibiotic levofloxacin (LVFX) alone or with the antibody was also studied. Protection included an increase in survival rate. Treatment with the anti-TNF-α antibody inhibited the histological signs associated with EHEC infection but did not prevent the colonization of EHEC or production of Shiga toxin (Stx). No clinical signs were observed and EHEC was completely eliminated in the mouse model receiving both anti-TNF-α antibody and LVFX. Anti-TNF-α antibody suppressed inflammatory cytokine response in the mouse kidney and brain by EHEC infection.

Original languageEnglish
Pages (from-to)217-231
Number of pages15
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume24
Issue number4
DOIs
Publication statusPublished - 2001 Jan 1

Keywords

  • Anti-TNF-α antibody
  • Escherichia coli 0157
  • Levofloxacin
  • Mouse model
  • Shiga toxin

ASJC Scopus subject areas

  • Microbiology
  • Immunology and Allergy
  • Immunology
  • veterinary(all)
  • Infectious Diseases

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