Theonezolide A, a novel marine macrolide, induces drastic shape change in rabbit platelets by reorganization of microtubules

Minori Mitsui-Saito, Satoko Ohkubo, Yutaro Obara, Teruyuki Yanagisawa, Jun'Ichi Kobayashi, Yasushi Ohizumi, Norimichi Nakahata

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Theonezolide A, a marine macrolide, and thrombin caused a shape change followed by an aggregation in the rabbit platelets. Theonezolide A-induced platelet shape change, estimated by a decrease in light transmission, appeared to a greater extent than thrombin-induced one. Morphological studies using an electron microscope showed that theonezolide A changed platelet shape with various numbers of long pseudopods, loosing their discoid shape. Theonezolide A-induced shape change was inhibited by a microtubule-stabilizing agent, taxol, but not by an actin-depolymerizing agent, cytochalasin B. In contrast, thrombin-induced shape change was inhibited by cytochalasin B but not by taxol. Confocal fluorescence microscopy showed that circumferential microtubule bundle disappeared in the platelets treated with theonezolide A. Theonezolide A had no direct effect on polymerization of microtubules isolated from bovine brain, indicating that it indirectly causes microtubule reorganization. These results suggest that theonezolide A induces drastic shape change through reorganization of microtubules in rabbit platelets. Thus, theonezolide A is a useful drug to examine microtubule reorganization in the cells.

Original languageEnglish
Pages (from-to)133-138
Number of pages6
JournalThrombosis Research
Volume108
Issue number2-3
DOIs
Publication statusPublished - 2002 Nov 1

Keywords

  • Actin
  • Platelet activation
  • Taxol
  • Theonezolide A
  • Tubulin

ASJC Scopus subject areas

  • Hematology

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