The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A

Elsa Kress, Françoise Schwager, René Holtackers, Jonas Seiler, François Prodon, Esther Zanin, Annika Eiteneues, Mika Toya, Asako Sugimoto, Hemmo Meyer, Patrick Meraladi, Monica Gotta

    Research output: Contribution to journalArticlepeer-review

    15 Citations (Scopus)

    Abstract

    Coordination of cell cycle events in space and time is crucial to achieve a successful cell division. Here, we demonstrate that UBXN-2, a substrate adaptor of the AAA ATPase Cdc48/p97, is required to coordinate centrosome maturation timing with mitosis. In UBXN-2-depleted Caenorhabditis elegans embryos, centrosomes recruited more AIR-1 (Aurora A), matured precociously, and alignment of the mitotic spindle with the axis of polarity was impaired. UBXN-2 and CDC-48 coimmunoprecipitated with AIR-1 and the spindle alignment defect was partially rescued by co-depleting AIR-1, indicating that UBXN-2 controls these processes via AIR-1. Similarly, depletion in human cells of the UBXN-2 orthologues p37/p47 resulted in an accumulation of Aurora A at centrosomes and a delay in centrosome separation. The latter defect was also rescued by inhibiting Aurora A. We therefore postulate that the role of this adaptor in cell cycle regulation is conserved.

    Original languageEnglish
    Pages (from-to)559-575
    Number of pages17
    JournalJournal of Cell Biology
    Volume201
    Issue number4
    DOIs
    Publication statusPublished - 2013 May

    ASJC Scopus subject areas

    • Cell Biology

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