The Transport of Prolamine RNAs to Prolamine Protein Bodies in Living Rice Endosperm Cells

Shigeki Hamada, Keiki Ishiyama, Sang Bong Choi, Changlin Wang, Salvinder Singh, Naoko Kawai, Vincent R. Franceschi, Thomas W. Okita

Research output: Contribution to journalArticlepeer-review

62 Citations (Scopus)

Abstract

RNAs that code for the major rice storage proteins are localized to specific subdomains of the cortical endoplasmic reticulum (ER) in developing endosperm. Prolamine RNAs are localized to the ER and delimit the prolamine intracisternal inclusion granules (PB-ER), whereas glutelin RNAs are targeted to the cisternal ER. To study the transport of prolamine RNAs to the surface of the prolamine protein bodies in living endosperm cells, we adapted a two-gene system consisting of green fluorescent protein (GFP) fused to the viral RNA binding protein MS2 and a hybrid prolamine RNA containing tandem MS2 RNA binding sites. Using laser scanning confocal microscopy, we show that the GFP-labeled prolamine RNAs are transported as particles that move at an average speed of 0.3 to 0.4 μm/s. These prolamine RNA transport particles generally move unidirectionally in a stop-and-go manner, although nonlinear bidirectional, restricted, and nearly random movement patterns also were observed. Transport is dependent on intact microfilaments, because particle movement is inhibited rapidly by the actin filament-disrupting drugs cytochalasin D and latrunculin B. Direct evidence was obtained that these prolamine RNA-containing particles are transported to the prolamine protein bodies. The significance of these results with regard to protein synthesis in plants is discussed.

Original languageEnglish
Pages (from-to)2253-2264
Number of pages12
JournalPlant Cell
Volume15
Issue number10
DOIs
Publication statusPublished - 2003 Oct
Externally publishedYes

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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