The TLR Expression Pattern on Monocyte-Derived Macrophages for Lipopolysaccharid Stimulation of Calves

Yi jie GUO, Guo Qi ZHAO, Yong jiu HUO, Sachi Tanaka, Hisashi Aso, Takahiro Yamaguchi

Research output: Contribution to journalArticlepeer-review

Abstract

In this paper, toll-like receptor expression pattern in monocytes-derived macrophages by lipopolysaccharid (LPS) stimulation was examined. Jugular venous blood samples from 4 Japanese calves were obtained and the peripheral blood mononuclear cells (PBMC) were isolated. The PBMC were cultured for 7 d so as to collect monocytes-derived macrophages in Repcell. The PBMC were stimulated by LPS for 24 h and the mRNA expression pattern of TLR and cytokines in monocytes-derived macrophages (Mod-Mφ{symbol}) was analyzed. Results showed that LPS stimulation of Mod-Mφ{symbol} could increase the mRNA levels of the genes of TNF-α, IL-6, and IL-8. In addition, the mRNA levels of the genes of TNF-α and IL-6 in the group of LPS stimulation were most significantly (P < 0.01) higher than those in control group and the mRNA levels of TLR1, 3, 5, 8, and 10 were significantly (P < 0.05) decreased after LPS stimulation. There was no difference in the mRNA expressions of TLR2, 4, 6, and 7 between the groups of the control and LPS stimulation. Besides, expression of TLR9 was not found. It suggested that monocytes-derived macrophages could respond to LPS and they might take an important role in the innate immunity. The important function of the cells might contribute to better disease treatment.

Original languageEnglish
Pages (from-to)864-871
Number of pages8
JournalAgricultural Sciences in China
Volume8
Issue number7
DOIs
Publication statusPublished - 2009 Jul

Keywords

  • LPS
  • TLR
  • bovine
  • macrophage

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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