The thymus atrophy-inducing organotin compound dbtc inhibits the binding of thymocytes to thymic epithelial cells

Raymond H.H. Pieters, Ruud Albers, Rob Bleumink, Niek J. Snoeij, Tsunetoshi Itoh, Willem Seinen, Andr ÉH Penninks

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In this study, it is examined whether the organotin compound di-n-butyltindichloride (DBTC), which has been shown to inhibit immature thymocyte proliferation, is able to disturb the binding between thymocytes and thymic epithelial cells (TEC). To that end, an enzyme-linked binding assay was developed in which the amount of binding of Thy-1+ (mAb ER4)-thymocytes to the rat-derived TEC-line IT45R1 (IT45R1-TEC) could be detected. It was found that preincubation of thymocytes with 3-5 μM DBTC for 30 min inhibited the binding by 50-60% during a 1 h adhesion period. By extending the preincubation period to 1 h and the adhesion period to 22 h, 0.1 μM DBTC was already sufficient to reduce the binding with 60-80%. Further characterization of the binding revealed that splenic lymphocytes were unable to bind to the MHC class II-negative IT45R1-TEC. Since dextran sulfate inhibited the binding as well, sulfated polysaccharide-binding molecules such as Thy-I and CD2 are likely to be involved in the binding. Electron microscopy showed filament-containing microvilli at the site of interaction. The results are discussed in relation to the mechanism of DBTC-induced thymus atrophy.

Original languageEnglish
Pages (from-to)329-333,335-337
JournalInternational Journal of Immunopharmacology
Volume17
Issue number4
DOIs
Publication statusPublished - 1995 Apr

Keywords

  • cell contact
  • organotins
  • thymic epithelial cells
  • thymus atrophy

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Fingerprint Dive into the research topics of 'The thymus atrophy-inducing organotin compound dbtc inhibits the binding of thymocytes to thymic epithelial cells'. Together they form a unique fingerprint.

  • Cite this

    Pieters, R. H. H., Albers, R., Bleumink, R., Snoeij, N. J., Itoh, T., Seinen, W., & Penninks, A. ÉH. (1995). The thymus atrophy-inducing organotin compound dbtc inhibits the binding of thymocytes to thymic epithelial cells. International Journal of Immunopharmacology, 17(4), 329-333,335-337. https://doi.org/10.1016/0192-0561(94)00103-U