The T3b gene promoter directs intestinal epithelial cell-specific expression in transgenic mice

Hiroyuki Aihara, Nobuo Hiwatashi, Shinji Kumagai, Yuhichi Obata, Tooru Shimosegawa, Takayoshi Toyota, Jun Ichi Miyazaki

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Although a few promoters that direct intestinal epithelial cell-specific expression in transgenic animals have been reported, they are not necessarily appropriate for transgenic studies in terms of activity and tissue specificity. Here, we examined the tissue specificity of transgene expression directed by the 2.8-kb promoter region of the T3b gene, which encodes one of the non-classical major histocompatibility complex class I molecules. The transgene was expressed exclusively in the epithelial cells of the small and large intestines at high levels. The results indicate that the T3b promoter is useful for directing transgene expression specifically in intestinal epithelial cells.

Original languageEnglish
Pages (from-to)185-188
Number of pages4
JournalFEBS Letters
Volume463
Issue number1-2
DOIs
Publication statusPublished - 1999 Dec 10

Keywords

  • Inflammatory bowel disease
  • Interleukin- 12
  • Tissue-specific promoter
  • Transgenic mouse

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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