The structure of the chloroplast genome in members of the genus Asparagus

Akira Kanno, Y. O. Lee, T. Kameya

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

In a previous study we constructed a physical map of the chloroplast DNA (ctDNA) of garden asparagus (Asparagus officinalis L. cv 'Mary Washington 500W'; Lee et al. 1996). In the present study we have constructed and compared HindIII and XhoI restriction maps of the ctDNAs of eight species of Asparagus: namely, A. officinalis, A. schoberioides, A. coehinchinensis, A. plumosus, A. falcatus, A. sprengeri, A. virgatus and A. asparagoides. The ctDNA of A. officinalis has 32 and 23 sites that are recognized by HindIII and XhoI, respectively. Taking the physical map of the ctDNA of A. officinalis as a standard, we found that the ctDNAs of A. falcatus, A. sprengeri, and A. asparagoides each had one additional HindIII site and lacked one XhoI site. We also detected two relatively large deletions of nucleotides in the ctDNA from A. cochinchinensis by sequencing analysis. Both of these deletions were located in a non-coding region between the ndhC and trnV genes and were 95 bp and 347 bp in length, respectiveIy. The regions around the deletions exhibited strong homology, and short direct-repeat sequences were detected at the borders of the deletions, an indication that these deletions were the result of intramolecular recombination mediated by the direct repeats.

Original languageEnglish
Pages (from-to)1196-1202
Number of pages7
JournalTheoretical and Applied Genetics
Volume95
Issue number8
DOIs
Publication statusPublished - 1997 Dec 1

Keywords

  • Asparagus
  • Chloroplast DNA (ctDNA)
  • Deletion
  • Direct repeat

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Plant Science
  • Genetics
  • Genetics(clinical)

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