The Structure-Function Relationship of GLP-1 Related Peptides in the Endocrine Function of the Canine Pancreas

Akira Ohneda, Kinuko Ohneda, Makoto Ohneda, Fumiaki Koizumi, Shinichi Ohashi, Koichi Kawai, Seiji Suzuki

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

In order to clarify the relationship between the structure and function of glucagon-like peptide (GLP) 1 in the endocrine function of the pancreas, the response of insulin and glucagon to various synthetic GLP-1-related peptides was investigated in anesthetized dogs. GLP-1-related peptides were administered in a dosage of 400 pmol within 10 min into the pancreatic artery during glucose or arginine infusion and the changes in plasma insulin and glucagon in the pancreatic vein were studied. GLP-1 (7-36) and (7-37), as well as glucagon enhanced insulin release during glucose infusion, whereas neither GLP-1 (1-37), (7-20), (6-37) nor (8-37) stimulated insulin release. The administration of GLP-1 (1-37), (7-36) and (7-37) reduced glucagon release during glucose infusion. When arginine was infused, GLP-1 (7-20), (7-36), (7-37), and glucagon enhanced insulin release. In contrast, glucagon release was increased by the administration of GLP-1 (7-20), (8-37), and (7-37). The present study indicates that histidine at the 7th position of GLP-1 is important in eliciting biological action and that only truncated GLP-1 (7-36), (7-37), and (7-20) showed an insulinotropic action as strong as glucagon in dogs. Furthermore, it is suggested that the response of insulin and glucagon to GLP-1-related peptides is dependent on a background condition.

Original languageEnglish
Pages (from-to)209-221
Number of pages13
JournalTohoku Journal of Experimental Medicine
Volume165
Issue number3
DOIs
Publication statusPublished - 1991

Keywords

  • glucagon
  • insulin
  • local circulation of pancreas

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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