The Sperm TRP-3 Channel Mediates the Onset of a Ca2+ Wave in the Fertilized C. elegans Oocyte

Jun Takayama, Shuichi Onami

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Sperm induce Ca2+ waves in the fertilized egg by introducing soluble factors or by surface interactions, which activate egg Ca2+ channels. Involvement of sperm Ca2+ channels is predicted by the conduit model; however, this model has not been validated. In Caenorhabditis elegans, the sperm-specific TRP family Ca2+ channel TRP-3 mediates sperm-oocyte fusion. Here, using high-speed in vivo imaging and image analyses, we show that sperm induce an immediate local Ca2+ rise followed by a Ca2+ wave in fertilized C. elegans oocytes. Oocytes fertilized by rare trp-3 escaper sperm showed a lack of local rise and a delay in onset of the Ca2+ wave. Sperm Ca2+ imaging suggests that the local rise is not due to the bolus introduction of stored Ca2+. These results suggest that, along with its primary function in sperm-oocyte fusion, TRP-3 induces Ca2+ waves in fertilized oocytes, consistent with the conduit model.

Original languageEnglish
Pages (from-to)625-637
Number of pages13
JournalCell Reports
Volume15
Issue number3
DOIs
Publication statusPublished - 2016 Apr 19
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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