The small GTPase Rab33A participates in regulation of amylase release from parotid acinar cells

Akane Imai, Maiko Tsujimura, Sumio Yoshie, Mitsunori Fukuda

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    Amylase is released from exocrine parotid acinar cells via typical exocytosis. Exocytosis of amylase-containing granules occurs through several steps, including formation, maturation, and transport of granules. These steps are thought to be regulated by members of the small GTPase Rab family. We previously demonstrated that Rab27 and its effectors mediate amylase release from parotid acinar cells, but the functional involvement of other Rab proteins in exocrine granule exocytosis remains largely unknown. Here, we studied isoproterenol (IPR)-induced amylase release from parotid acinar cells to investigate the possible involvement of Rab33A, which was recently suggested to regulate exocytosis in hippocampal neurons and PC12 cells. Rab33A was endogenously expressed in parotid acinar cells and present in secretory granules and the Golgi body. Functional ablation of Rab33A with anti-Rab33A antibody or a dominant-negative Rab33A-T50N mutant significantly reduced IPR-induced amylase release. Our results indicated that Rab33A is a novel component of IPR-stimulated amylase secretion from parotid acinar cells.

    Original languageEnglish
    Pages (from-to)469-474
    Number of pages6
    JournalBiochemical and biophysical research communications
    Volume461
    Issue number3
    DOIs
    Publication statusPublished - 2015 Jun 5

    Keywords

    • Amylase release
    • Intracellular distribution
    • Parotid
    • Rab33A
    • β-stimulation

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Molecular Biology
    • Cell Biology

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