The small GTPase Rab27B regulates amylase release from rat parotid acinar cells

Akane Imai, Sumio Yoshie, Tomoko Nashida, Hiromi Shimomura, Mitsunori Fukuda

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Small GTPase Rab is a large family of putative membrane trafficking proteins, and each member is thought to regulate a specific type(s) of membrane trafficking. However, little is known about the involvement of Rab protein(s) in secretory granule exocytosis in exocrine cells or the molecular mechanism underlying this process. We show that Rab27B, a closely related isoform of Rab27A that regulates lysosome-related granule exocytosis in cytotoxic T lymphocytes, is abundantly expressed on amylase-containing secretory granules in rat parotid gland acinar cells. We also identify the putative Rab27B effector protein, Slac2-c (Slp homologue lacking C2 domains-c)/MyRIP, which was originally described as a myosin Va/VIIa and actin binding protein, in rat parotid glands. The results of subcellular fractionation, immunoprecipitation and immunohistochemical studies indicate that the Rab27B-Slac2-c complex is formed on secretory granules in vivo. The introduction of either a specific Rab27 binding domain (i.e. a recombinant Slp homology domain of Slac2-b that specifically binds Rab27A/B but not other Rabs) or functionally blocking antibodies that specifically disrupt Rab27B-Slac2-c complex in vitro strongly inhibited isoproterenol-stimulated amylase release from streptolysin O-permeabilized parotid acinar cells. Our results indicate that the Rab27B-Slac2-c complex is an important constituent of secretory granule exocytosis in parotid acinar cells.

Original languageEnglish
Pages (from-to)1945-1953
Number of pages9
JournalJournal of cell science
Volume117
Issue number10
DOIs
Publication statusPublished - 2004 Apr 15
Externally publishedYes

Keywords

  • Griscelli syndrome
  • Parotid gland
  • Rab27B
  • Secretory granule exocytosis
  • Slac2-c/MyRIP

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'The small GTPase Rab27B regulates amylase release from rat parotid acinar cells'. Together they form a unique fingerprint.

  • Cite this