The SM protein VPS-45 is required for RAB-5-dependent endocytic transport in Caenorhabditis elegans

Keiko Gengyo-Ando, Hidehito Kuroyanagi, Tetsuo Kobayashi, Motohide Murate, Kazushi Fujimoto, Shigeo Okabe, Shohei Mitani

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

Rab5, a small guanosine triphosphatase, is known to regulate the tethering and docking reaction leading to SNARE (soluble NSF attachment protein receptors)-mediated fusion between endosomes. However, it is uncertain how the signal of the activated Rab5 protein is transduced by its downstream effectors during endosome fusion. Here, we show that the Sec1/Munc18 gene vps-45 is essential for not only viability and development but also receptor-mediated and fluid-phase endocytosis pathways in Caenorhabditis elegans. We found that VPS-45 interacts with a Rab5 effector, Rabenosyn-5 (RABS-5), and the mutants of both vps-45 and rabs-5 show similar endocytic phenotypes. In the macrophage-like cells of vps-45 and rabs-5 mutants, aberrantly small endosomes were accumulated, and the endosome fusion stimulated by the mutant RAB-5 (Q78L) is suppressed by these mutations. Our results indicate that VPS-45 is a key molecule that functions downstream from RAB-5, cooperating with RABS-5, to regulate the dynamics of the endocytic system in multicellular organisms.

Original languageEnglish
Pages (from-to)152-157
Number of pages6
JournalEMBO Reports
Volume8
Issue number2
DOIs
Publication statusPublished - 2007 Feb

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'The SM protein VPS-45 is required for RAB-5-dependent endocytic transport in Caenorhabditis elegans'. Together they form a unique fingerprint.

Cite this