The shape of the sperm midpiece in intracytoplasmic morphologically selected sperm injection relates sperm centrosomal function

Tomohisa Ugajin, Yukihiro Terada, Hisataka Hasegawa, Hiroshi Nabeshima, Kichiya Suzuki, Nobuo Yaegashi

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Purpose: To evaluate whether the morphology of the sperm midpiece observed by high magnification microscopy relates to sperm centrosomal function. Methods: Sperm selected by conventional microscopy were defined as controls. By high magnification microscopy, sperm with straight midpieces were defined as Group 1, while those with tapering midpieces were defined as Group 2. Heterologous ICSI of human sperm into bovine oocytes was used to assess human sperm centrosomal function and analysis of sperm aster formation. Results: The total rate of sperm aster formation was 80.5% in Group 1, which was significantly higher (P∈<∈0.05) than the rate of 33.3% seen for Group 2. Furthermore, sperm aster formation rates tended to be higher for Group 1 than for the controls. Conclusions: This study demonstrates improvement of sperm aster formation rates by selecting sperm on the basis of midpiece morphology. The injection of selected sperm bearing morphologically straight midpieces may contribute to improved expression of sperm centrosomal function, providing a positive effect on fertilization after ICSI.

Original languageEnglish
Pages (from-to)75-81
Number of pages7
JournalJournal of Assisted Reproduction and Genetics
Volume27
Issue number2-3
DOIs
Publication statusPublished - 2010 Feb

Keywords

  • Centrosomal function
  • Intracytoplasmic morphologically selected sperm injection (IMSI)
  • Sperm aster formation
  • Sperm midpiece

ASJC Scopus subject areas

  • Reproductive Medicine
  • Genetics
  • Obstetrics and Gynaecology
  • Developmental Biology
  • Genetics(clinical)

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